Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human
Western blot analysis of various lysates, using Phospho-IP3R-S1756 Rabbit pAb (AP1224) at 1:400 dilution. HeLa cells were treated by Forskolin (10 uM) at 37℃ for 1 hour after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
| Product name | Phospho-IP3R-S1756 Rabbit pAb |
|---|---|
| Catalog No. | AP1224 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around S1756 of human ITPR1 (XP_011531986.1). |
|---|---|
| Sequence | RESLT |
| Gene ID | |
| Swiss Prot | |
| Synonyms | ACV; CLA4; IP3R; IP3R1; SCA15; SCA16; SCA29; INSP3R1; PPP1R94; Phospho-IP3R-S1756 |
| Calculated MW | 314kDa |
| Observed MW | 320kDa |
| Reactivity | Human |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | HeLa treated by Forskolin |
| Cellular location | endoplasmic reticulum, nucleolus, perinuclear region of cytoplasm, Schaffer collateral - CA1 synapse. |
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