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Islet1 Rabbit mAb (A0871)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Islet1 Rabbit mAb (A0871)

Western blot analysis of various lysates using Islet1 Rabbit mAb (A0871) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Islet1 Rabbit mAb (A0871)

Immunohistochemistry analysis of paraffin-embedded mouse fetal Heart using Islet1 Rabbit mAb (A0871) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameIslet1 Rabbit mAb
Catalog No.A0871
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0511
This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of human Islet1 (P61371).
SequenceYAANPRPDALMKEQLVEMTGLSPRVIRVWFQNKRCKDKKRSIMMKQLQQQQPNDKTNIQGMTGTPMVAASPERHDGGLQANPVEVQSYQPPWKVLSDFALQ
Gene ID
Swiss Prot
SynonymsIsl-1; ISLET1; Islet1
Calculated MW39kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, K-562, Rat pancreas
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - Islet1 Rabbit mAb (A0871)}

Western blot - Islet1 Rabbit mAb (A0871)

Western blot analysis of various lysates using Islet1 Rabbit mAb (A0871) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Islet1 Rabbit mAb (A0871)}

Immunohistochemistry - Islet1 Rabbit mAb (A0871)

Immunohistochemistry analysis of paraffin-embedded mouse fetal Heart using Islet1 Rabbit mAb (A0871) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A0871? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)