제품 > 항체 > 다중클론항체(pAb)

JMJD6 Rabbit pAb (A21829)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - JMJD6 Rabbit pAb (A21829)

Western blot analysis of lysates from Mouse liver using JMJD6 Rabbit pAb(A21829) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:90s.

ABclonal:Immunohistochemistry - JMJD6 Rabbit pAb (A21829)

Immunohistochemistry analysis of paraffin-embedded human lung using JMJD6 Rabbit pAb (A21829) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameJMJD6 Rabbit pAb
Catalog No.A21829
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a nuclear protein with a JmjC domain. JmjC domain-containing proteins are predicted to function as protein hydroxylases or histone demethylases. This protein was first identified as a putative phosphatidylserine receptor involved in phagocytosis of apoptotic cells; however, subsequent studies have indicated that it does not directly function in the clearance of apoptotic cells, and questioned whether it is a true phosphatidylserine receptor. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human JMJD6 (NP_055982.2).
SequenceMNHKSKKRIREAKRSARPELKDSLDWTRHNYYESFSLSPAAVADNVERADALQLSVEEFVERYERPYKPVVLLNAQEGWSAQEKWTLERLKRKYRNQKFKCGEDNDGYSVKMKMKYYIEYMESTRDDSPLYIFDSSYGEHPKRRKLLEDYKVPKFFTDDLFQYAGEKRRP
Gene ID
Swiss Prot
SynonymsPSR; PTDSR; PTDSR1; JMJD6
Calculated MW46kDa
Observed MW46kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse liver
Cellular locationNucleus, nucleolus, nucleoplasm

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ABclonal:Western blot - JMJD6 Rabbit pAb (A21829)}

Western blot - JMJD6 Rabbit pAb (A21829)

Western blot analysis of lysates from Mouse liver using JMJD6 Rabbit pAb(A21829) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:90s.
ABclonal:Immunohistochemistry - JMJD6 Rabbit pAb (A21829)}

Immunohistochemistry - JMJD6 Rabbit pAb (A21829)

Immunohistochemistry analysis of paraffin-embedded human lung using JMJD6 Rabbit pAb (A21829) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)