제품 > 항체 > 다중클론항체(pAb)

KDM7A Rabbit pAb (A8266)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using KDM7A Rabbit pAb (A8266) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Review

Overview

Product name	KDM7A Rabbit pAb
Catalog No.	A8266
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables histone H3-methyl-lysine-9 demethylase activity and histone H3-tri/di-methyl-lysine-27 demethylase activity. Involved in histone H3-K27 demethylation; histone H3-K9 demethylation; and positive regulation of transcription, DNA-templated. Predicted to be located in nucleolus and nucleoplasm. Human ortholog(s) of this gene implicated in melanoma. Orthologous to human KDM7A (lysine demethylase 7A).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 417-735 of mouse KDM7A (NP_001028602.2).
Sequence	LKEDGFQPQSYLVQGVKALHTALKLWMKKELVSEHAFEIPDNVRPGHLIKELSKVIRAIEEENGKPVKSQGIPSVCPVSRPSNEASPPYHSRRKMRKLRDHNVRTPSNLDILELHTREVLKRLEMCPWEEDLLSSKLNGKFNKHLQPSSTVPEWRAKDNDLRLLLTNGRIIKDERQLFADRSLYTADSENEEDKKPTQNANMKTEQSSGREEAESQGSPKPLNRIFTSVRSELRSRPSEYSDGSDSEDSGPDCTALKINFATEDSESSGDEKKHEITSHFKEESDIVRNLLQKSQKPSRQEIPVKRECPTSTSTEEEAIQGMLSMAGLHYSSCLQRQIQSTDCSGEKNSLQDPSSCHGSNPEFRQLYRCNKPVEFGYHAKTEDQDLMTSSWNKQFDRTSRFNAQDLSRSQKHIKKESSSEINQKAQSRHCVDSNSSSIQNGKYTLNPSLVSCQISNGSLSPERPIGETSFSMPLHPTKRPASNPPPISNQATKGKRPKKGMATAKQRLGKILKLNRNGHARFFV
Gene ID
Swiss Prot
Synonyms	Jhdm1d; mKIAA1718; A630082K20Rik; KDM7A
Calculated MW	92kDa/106kDa
Observed MW	106kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa, 293T, BT-474, Mouse brain, Mouse thymus, Mouse spleen, Rat brain, Rat thymus
Cellular location	Nucleus.
Customer validation	(Human hepatocellular carcinoma cell line) WB(Mus musculus)