제품 > 항체 > 단일클론항체(mAb)

[KD Validated] Bak Rabbit mAb (A23523)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - [KD Validated] Bak Rabbit mAb (A23523)

Western blot analysis of various lysates, using [KD Validated] Bak Rabbit mAb (A23523) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KD Validated] Bak Rabbit mAb (A23523)

Western blot analysis of various lysates, using [KD Validated] Bak Rabbit mAb (A23523) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KD Validated] Bak Rabbit mAb (A23523)

Western blot analysis of lysates from wild type (WT) and Bak knockdown (KD) 293T cells using [KD Validated] Bak Rabbit mAb (A23523) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - [KD Validated] Bak Rabbit mAb (A23523)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] Bak Rabbit mAb (A23523) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] Bak Rabbit mAb (A23523)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KD Validated] Bak Rabbit mAb (A23523) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] Bak Rabbit mAb (A23523)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] Bak Rabbit mAb (A23523) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] Bak Rabbit mAb (A23523)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] Bak Rabbit mAb (A23523) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] Bak Rabbit mAb (A23523)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] Bak Rabbit mAb (A23523) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product name[KD Validated] Bak Rabbit mAb
Catalog No.A23523
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC54625
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 11-82 of human Bak (NP_001179.1).
SequenceRQECGEPALPSASEEQVAQDTEEVFRSYVFYRHQQEQEAEGVAAPADPEMVTLPLQPSSTMGQVGRQLAIIG
Gene ID
Swiss Prot
SynonymsBAK; CDN1; BCL2L7; BAK-LIKE; Bak
Calculated MW23kDa
Observed MW23kDa/
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, THP-1, Molt-4, Mouse large intestine, Mouse small intestine, 293T
Cellular locationMitochondrion membrane, Single-pass membrane protein.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KD Validated] Bak Rabbit mAb (A23523)}

Western blot - [KD Validated] Bak Rabbit mAb (A23523)

Western blot analysis of various lysates, using [KD Validated] Bak Rabbit mAb (A23523) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KD Validated] Bak Rabbit mAb (A23523)}

Western blot - [KD Validated] Bak Rabbit mAb (A23523)

Western blot analysis of various lysates, using [KD Validated] Bak Rabbit mAb (A23523) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KD Validated] Bak Rabbit mAb (A23523)}

Western blot - [KD Validated] Bak Rabbit mAb (A23523)

Western blot analysis of lysates from wild type (WT) and Bak knockdown (KD) 293T cells using [KD Validated] Bak Rabbit mAb (A23523) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - [KD Validated] Bak Rabbit mAb (A23523)}

Immunohistochemistry - [KD Validated] Bak Rabbit mAb (A23523)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] Bak Rabbit mAb (A23523) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] Bak Rabbit mAb (A23523)}

Immunohistochemistry - [KD Validated] Bak Rabbit mAb (A23523)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KD Validated] Bak Rabbit mAb (A23523) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] Bak Rabbit mAb (A23523)}

Immunohistochemistry - [KD Validated] Bak Rabbit mAb (A23523)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] Bak Rabbit mAb (A23523) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] Bak Rabbit mAb (A23523)}

Immunohistochemistry - [KD Validated] Bak Rabbit mAb (A23523)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] Bak Rabbit mAb (A23523) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] Bak Rabbit mAb (A23523)}

Immunohistochemistry - [KD Validated] Bak Rabbit mAb (A23523)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] Bak Rabbit mAb (A23523) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)