Western blot - [KD Validated] COX7A2L Rabbit mAb (A25980)
Western blot analysis of lysates from Mouse heart using [KD Validated] COX7A2L Rabbit mAb (A25980) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Western blot - [KD Validated] COX7A2L Rabbit mAb (A25980)
Western blot analysis of lysates from wild type (WT) and COX7A2L knockdown (KD) HeLa cells using [KD Validated] COX7A2L Rabbit mAb (A25980) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Western blot - [KD Validated] COX7A2L Rabbit mAb (A25980)
Western blot analysis of various lysates using [KD Validated] COX7A2L Rabbit mAb (A25980) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry - [KD Validated] COX7A2L Rabbit mAb (A25980)
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] COX7A2L Rabbit mAb (A25980) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KD Validated] COX7A2L Rabbit mAb (A25980)
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KD Validated] COX7A2L Rabbit mAb (A25980) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KD Validated] COX7A2L Rabbit mAb (A25980)
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using [KD Validated] COX7A2L Rabbit mAb (A25980) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KD Validated] COX7A2L Rabbit mAb (A25980)
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] COX7A2L Rabbit mAb (A25980) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KD Validated] COX7A2L Rabbit mAb (A25980)
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] COX7A2L Rabbit mAb (A25980) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KD Validated] COX7A2L Rabbit mAb (A25980)
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KD Validated] COX7A2L Rabbit mAb (A25980) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.