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[KD Validated] FOSL1 Rabbit pAb (A21988)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - [KD Validated] FOSL1 Rabbit pAb (A21988)

Western blot analysis of extracts from wild type(WT) and FOSL1 knockdown (KD) U-87MG cells, using FOSL1 antibody (A21988) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product name[KD Validated] FOSL1 Rabbit pAb
Catalog No.A21988
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. Several transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human FOSL1 (NP_005429.1?).
SequenceMFRDFGEPGPSSGNGGGYGGPAQPPAAAQAAQQKFHLVPSINTMSGSQELQWMVQPHFLGPSSYPRPLTYPQYSPPQPRPGVIRALGPPPGVRRRPCEQI
Gene ID
Swiss Prot
SynonymsFRA; FRA1; fra-1
Calculated MW29kDa
Observed MW40kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG
Cellular locationNucleus
Customer validation

IF(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - [KD Validated] FOSL1 Rabbit pAb (A21988)}

Western blot - [KD Validated] FOSL1 Rabbit pAb (A21988)

Western blot analysis of extracts from wild type(WT) and FOSL1 knockdown (KD) U-87MG cells, using FOSL1 antibody (A21988) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)