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[KD Validated] GPX4 Rabbit pAb (A1933)

Review (1)Publications (134) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] GPX4 Rabbit pAb (A1933)

Western blot analysis of various lysates using [KD Validated] GPX4 Rabbit pAb (A1933) at 1:15000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KD Validated] GPX4 Rabbit pAb (A1933)

Western blot analysis of lysates from wild type (WT) and GPX4 knockdown (KD) U-87 MG cells using [KD Validated] GPX4 Rabbit pAb (A1933) at 1:15000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KD Validated] GPX4 Rabbit pAb (A1933)

Immunohistochemistry analysis of paraffin-embedded Human appendix tissue using GPX4 Rabbit pAb (A1933) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0.01 M Tris-EDTA repair solution (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] GPX4 Rabbit pAb (A1933)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using GPX4 Rabbit pAb (A1933) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0.01 M Tris-EDTA repair solution (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] GPX4 Rabbit pAb (A1933)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using GPX4 Rabbit pAb (A1933) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0.01 M Tris-EDTA repair solution (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KD Validated] GPX4 Rabbit pAb (A1933)

Immunofluorescence analysis of NIH/3T3 cells using GPX4 Rabbit pAb (A1933) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for [KD Validated] GPX4 Rabbit pAb(A1933)

Overview

Product name[KD Validated] GPX4 Rabbit pAb
Catalog No.A1933
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Transcript variants resulting from alternative splicing or use of alternate promoters have been described to encode isoforms with different subcellular localization.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 74-197 of GPX4 (NP_002076.2).
SequenceGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAAGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDLPHYF
Gene ID
Swiss Prot
SynonymsMCSP; SMDS; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx; GPX4
Calculated MW22kDa
Observed MW20kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:15000 - 1:60000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-87 MG, Jurkat, U-937, Mouse testis
Cellular locationCytoplasm, Mitochondrion.
Customer validation

(Mouse liver)

WB(Homo sapiens, Mus musculus, Gallus gallus, Rattus norvegicus, Rattus norvegicus, Danio rerio, Mus musculus, Ctenopharyngodon idellus)

IHC(Mus musculus, Rattus norvegicus, Homo sapiens)

IF(Danio rerio, Mus musculus, Cyprinus carpio, Gallus gallus, Rattus norvegicus, Capra hircus, Homo sapiens)

IHC(Rattus norvegicus, Homo sapiens)

IF(Homo sapiens, Mus musculus, Rattus norvegicus)

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

IP(Homo sapiens)

Biochemical detection(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KD Validated] GPX4 Rabbit pAb (A1933)}

Western blot - [KD Validated] GPX4 Rabbit pAb (A1933)

Western blot analysis of various lysates using [KD Validated] GPX4 Rabbit pAb (A1933) at 1:15000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KD Validated] GPX4 Rabbit pAb (A1933)}

Western blot - [KD Validated] GPX4 Rabbit pAb (A1933)

Western blot analysis of lysates from wild type (WT) and GPX4 knockdown (KD) U-87 MG cells using [KD Validated] GPX4 Rabbit pAb (A1933) at 1:15000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KD Validated] GPX4 Rabbit pAb (A1933)}

Immunohistochemistry - [KD Validated] GPX4 Rabbit pAb (A1933)

Immunohistochemistry analysis of paraffin-embedded Human appendix tissue using GPX4 Rabbit pAb (A1933) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0.01 M Tris-EDTA repair solution (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] GPX4 Rabbit pAb (A1933)}

Immunohistochemistry - [KD Validated] GPX4 Rabbit pAb (A1933)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using GPX4 Rabbit pAb (A1933) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0.01 M Tris-EDTA repair solution (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] GPX4 Rabbit pAb (A1933)}

Immunohistochemistry - [KD Validated] GPX4 Rabbit pAb (A1933)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using GPX4 Rabbit pAb (A1933) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0.01 M Tris-EDTA repair solution (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KD Validated] GPX4 Rabbit pAb (A1933)}

Immunofluorescence - [KD Validated] GPX4 Rabbit pAb (A1933)

Immunofluorescence analysis of NIH/3T3 cells using GPX4 Rabbit pAb (A1933) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (3)

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재조합 단백질 (1)

Recombinant Human GPX4 Protein
RP02834

Tags:N-His

Expressed Host:E. coli

Recombinant Human GPX4 Protein

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)