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Phospho-Akt-S473 Rabbit mAb (AP1208)

Publications (35) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-Akt-S473 Rabbit mAb (AP1208)

Western blot analysis of various lysates using Phospho-Akt-S473 Rabbit mAb (AP1208) at 1:1000 dilution. Both Jurkat and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Phospho-Akt-S473 Rabbit mAb (AP1208)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Akt-S473 Rabbit mAb (AP1208) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product namePhospho-Akt-S473 Rabbit mAb
Catalog No.AP1208
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC5023-06
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
ImmunogenA synthetic phosphorylated peptide around S473 of human AKT1 (NP_005154.2).
SequenceQFSYS
Gene ID
Swiss Prot
SynonymsAKT1/AKT2/AKT3; Phospho-Akt-S473
Calculated MW48kDa/55kDa/51kDa/54kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesJurkat treated by Calyculin A, NIH/3T3 treated by Calyculin A, C6 treated by Calyculin A
Cellular locationciliary basal body, cytoplasm, cytosol, microtubule cytoskeleton, mitochondrion, nucleoplasm, nucleus, plasma membrane, spindle.
Customer validation

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Oryctolagus cuniculus, Sus scrofa)

IHC(Oryctolagus cuniculus)

IF(Oryctolagus cuniculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Phospho-Akt-S473 Rabbit mAb (AP1208)}

    Western blot - Phospho-Akt-S473 Rabbit mAb (AP1208)

    Western blot analysis of various lysates using Phospho-Akt-S473 Rabbit mAb (AP1208) at 1:1000 dilution. Both Jurkat and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - Phospho-Akt-S473 Rabbit mAb (AP1208)}

    Western blot - Phospho-Akt-S473 Rabbit mAb (AP1208)

    Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Akt-S473 Rabbit mAb (AP1208) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AP1208? Please let us know so that we can cite the reference in this datasheet.

    항체 (9)

    Secondary Antibodies (26)