Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from C6 cells using Phospho-Akt-S473 Rabbit pAb (AP1068) at 1:500 dilution. C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of lysates from NIH/3T3 cells using Phospho-Akt-S473 Rabbit pAb (AP1068) at 1:500 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
Western blot analysis of lysates from Jurkat cells using Phospho-Akt-S473 Rabbit pAb (AP1068) at 1:500 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Rat lung using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Rabbit pAb (AP1068) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Rabbit pAb (AP1068) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
| Product name | Phospho-Akt-S473 Rabbit pAb |
|---|---|
| Catalog No. | AP1068 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around S473 of human AKT1/AKT2/AKT3AKT1 (NP_005154.2). |
|---|---|
| Sequence | QFSYS |
| Gene ID | |
| Swiss Prot | |
| Synonyms | AKT1/AKT2/AKT3; Phospho-Akt-S473 |
| Calculated MW | 48kDa/55kDa/51kDa/54kDa |
| Observed MW | 60kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry |
| Positive samples | NIH/3T3 treated by Calyculin A, C6 treated by Calyculin A, Jurkat treated by Calyculin A |
| Cellular location | ciliary basal body, cytoplasm, cytosol, microtubule cytoskeleton, mitochondrion, nucleoplasm, nucleus, plasma membrane, spindle. |
| Customer validation | WB(Mus musculus, Gallus gallus, Homo sapiens, Rattus norvegicus) IHC(Mus musculus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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