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Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb (AP1430)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from RD cells, using Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb (AP1430) at 1:10000 dilution.RD cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from C2C12 cells, using Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb (AP1430) at 1:10000 dilution.C2C12 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb (AP1430)

Immunohistochemistry analysis of Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 in paraffin-embedded Rat kidney tissue using Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb (AP1430) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 in paraffin-embedded Human tonsil tissue using Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb (AP1430) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 in paraffin-embedded Mouse intestin tissue using Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb (AP1430) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 in paraffin-embedded Rat intestine tissue using Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb (AP1430) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 in paraffin-embedded Mouse kidney tissue using Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb (AP1430) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 in paraffin-embedded Human breast tissue using Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb (AP1430) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product name	Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb
Catalog No.	AP1430
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC61098

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T450 of human Akt.
Sequence	TITPP
Gene ID
Swiss Prot
Synonyms
Calculated MW	48KDa/51KDa/55KDa
Observed MW	60kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:10000 IHC-P 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	RD, C2C12
Cellular location	Cell membrane, Cytoplasm, Nucleus