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[KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706)

Western blot analysis of lysates from A-549 cells, using [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706)

Western blot analysis of lysates from wild type(WT) and Integrin alpha 5 (ITGA5/CD49e) knockdown (KD) HeLa cells, using [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product name[KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb
Catalog No.A22706
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC58291
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 42-995 of human Integrin alpha 5 (ITGA5/CD49e). (NP_002196.4).
SequenceFNLDAEAPAVLSGPPGSFFGFSVEFYRPGTDGVSVLVGAPKANTSQPGVLQGGAVYLCPWGASPTQCTPIEFDSKGSRLLESSLSSSEGEEPVEYKSLQWFGATVRAHGSSILACAPLYSWRTEKEPLSDPVGTCYLSTDNFTRILEYAPCRSDFSWAAGQGYCQGGFSAEFTKTGRVVLGGPGSYFWQGQILSATQEQIAESYYPEYLINLVQGQLQTRQASSIYDDSYLGYSVAVGEFSGDDTEDFVAGVPKGNLTYGYVTILNGSDIRSLYNFSGEQMASYFGYAVAATDVNGDGLDDLLVGAPLLMDRTPDGRPQEVGRVYVYLQHPAGIEPTPTLTLTGHDEFGRFGSSLTPLGDLDQDGYNDVAIGAPFGGETQQGVVFVFPGGPGGLGSKPSQVLQPLWAASHTPDFFGSALRGGRDLDGNGYPDLIVGSFGVDKAVVYRGRPIVSASASLTIFPAMFNPEERSCSLEGNPVACINLSFCLNASGKHVADSIGFTVELQLDWQKQKGGVRRALFLASRQATLTQTLLIQNGAREDCREMKIYLRNESEFRDKLSPIHIALNFSLDPQAPVDSHGLRPALHYQSKSRIEDKAQILLDCGEDNICVPDLQLEVFGEQNHVYLGDKNALNLTFHAQNVGEGGAYEAELRVTAPPEAEYSGLVRHPGNFSSLSCDYFAVNQSRLLVCDLGNPMKAGASLWGGLRFTVPHLRDTKKTIQFDFQILSKNLNNSQSDVVSFRLSVEAQAQVTLNGVSKPEAVLFPVSDWHPRDQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTRVTGLNCTTNHPINPKGLELDPEGSLHHQQKREAPSRSSASSGPQILKCPEAECFRLRCELGPLHQQESQSLQLHFRVWAKTFLQREHQPFSLQCEAVYKALKMPYRILPRQLPQKERQVATAVQWTKAEGSY
Gene ID
Swiss Prot
SynonymsFNRA; CD49e; VLA-5; VLA5A; [KD Validated] Integrin alpha 5 (ITGA5/CD49e)
Calculated MW115kDa
Observed MW150kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, A-549
Cellular locationMembrane, Single-pass type I membrane protein

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706)}

Western blot - [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706)

Western blot analysis of lysates from A-549 cells, using [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706)}

Western blot - [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706)

Western blot analysis of lysates from wild type(WT) and Integrin alpha 5 (ITGA5/CD49e) knockdown (KD) HeLa cells, using [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (7)

ELISA 키트 (1)

Secondary Antibodies (26)