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[KD Validated] MCM6 Rabbit mAb (A21112)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - [KD Validated] MCM6 Rabbit mAb (A21112)

Western blot analysis of various lysates using [KD Validated] MCM6 Rabbit mAb (A21112) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - [KD Validated] MCM6 Rabbit mAb (A21112)

Western blot analysis of lysates from wild type (WT) and MCM6 knockdown (KD) 293T cells, using [KD Validated] MCM6 Rabbit mAb (A21112) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product name[KD Validated] MCM6 Rabbit mAb
Catalog No.A21112
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC3057
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 601-821 of human MCM6 (NP_005906.2).
SequenceLRQRDGSGVTKSSWRITVRQLESMIRLSEAMARMHCCDEVQPKHVKEAFRLLNKSIIRVETPDVNLDQEEEIQMEVDEGAGGINGHADSPAPVNGINGYNEDINQESAPKASLRLGFSEYCRISNLIVLHLRKVEEEEDESALKRSELVNWYLKEIESEIDSEEELINKKRIIEKVIHRLTHYDHVLIELTQAGLKGSTEGSESYEEDPYLVVNPNYLLED
Gene ID
Swiss Prot
SynonymsMis5; P105MCM; MCG40308; [KO/KD Validated] MCM6
Calculated MW93kDa
Observed MW93kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, HeLa, Jurkat, Mouse testis
Cellular locationNucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - [KD Validated] MCM6 Rabbit mAb (A21112)}

    Western blot - [KD Validated] MCM6 Rabbit mAb (A21112)

    Western blot analysis of various lysates using [KD Validated] MCM6 Rabbit mAb (A21112) at1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Western blot - [KD Validated] MCM6 Rabbit mAb (A21112)}

    Western blot - [KD Validated] MCM6 Rabbit mAb (A21112)

    Western blot analysis of lysates from wild type (WT) and MCM6 knockdown (KD) 293T cells, using [KD Validated] MCM6 Rabbit mAb (A21112) at1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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