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MCM6 Rabbit pAb (A1955)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MCM6 Rabbit pAb (A1955)

Western blot analysis of various lysates using MCM6 Rabbit pAb (A1955) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - MCM6 Rabbit pAb (A1955)

Immunofluorescence analysis of U2OS cells using MCM6 Rabbit pAb (A1955).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

ABclonal:Immunoprecipitation - MCM6 Rabbit pAb (A1955)

Immunoprecipitation analysis of 300 μg extracts of Hela cells using 3 μg MCM6 antibody (A1955). Western blot was performed from the immunoprecipitate using MCM6 antibody (A1955) at a dilution of 1:1000.

Overview

Product nameMCM6 Rabbit pAb
Catalog No.A1955
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 562-821 of human MCM6 (NP_005906.2).
SequenceSIDRVYSLDDIRRYLLFARQFKPKISKESEDFIVEQYKHLRQRDGSGVTKSSWRITVRQLESMIRLSEAMARMHCCDEVQPKHVKEAFRLLNKSIIRVETPDVNLDQEEEIQMEVDEGAGGINGHADSPAPVNGINGYNEDINQESAPKASLRLGFSEYCRISNLIVLHLRKVEEEEDESALKRSELVNWYLKEIESEIDSEEELINKKRIIEKVIHRLTHYDHVLIELTQAGLKGSTEGSESYEEDPYLVVNPNYLLED
Gene ID
Swiss Prot
SynonymsMis5; P105MCM; MCG40308; MCM6
Calculated MW93kDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesU-251MG, HeLa, MCF7, HL-60, NIH/3T3, Mouse spleen
Cellular locationNucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - MCM6 Rabbit pAb (A1955)}

    Western blot - MCM6 Rabbit pAb (A1955)

    Western blot analysis of various lysates using MCM6 Rabbit pAb (A1955) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - MCM6 Rabbit pAb (A1955)}

    Immunofluorescence - MCM6 Rabbit pAb (A1955)

    Immunofluorescence analysis of U2OS cells using MCM6 Rabbit pAb (A1955).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.
    ABclonal:Immunoprecipitation - MCM6 Rabbit pAb (A1955)}

    Immunoprecipitation - MCM6 Rabbit pAb (A1955)

    Immunoprecipitation analysis of 300 μg extracts of Hela cells using 3 μg MCM6 antibody (A1955). Western blot was performed from the immunoprecipitate using MCM6 antibody (A1955) at a dilution of 1:1000.

    * For research use only. Not for therapeutic or diagnostic purposes.

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