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[KD Validated] METTL3 Rabbit mAb (A26858)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] METTL3 Rabbit mAb (A26858)

Western blot analysis of various lysates using [KD Validated] METTL3 Rabbit mAb (A26858) at 1:5000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KD Validated] METTL3 Rabbit mAb (A26858)

Western blot analysis of various lysates using [KD Validated] METTL3 Rabbit mAb (A26858) at 1:5000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KD Validated] METTL3 Rabbit mAb (A26858)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using [KD Validated] METTL3 Rabbit mAb (A26858) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunoprecipitation - [KD Validated] METTL3 Rabbit mAb (A26858)

Immunoprecipitation of METTL3 from 300 µg extracts of HepG2 cells was performed using 0.5 µg of [KD Validated] METTL3 Rabbit mAb (A26858). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using [KD Validated] METTL3 Rabbit mAb (A26858) at a dilution of 1 : 1000.

ABclonal:Immunoprecipitation - [KD Validated] METTL3 Rabbit mAb (A26858)

Immunoprecipitation of METTL3 from 300 µg extracts of HepG2 cells was performed using 0.5 µg of METTL3 Rabbit mAb (A26858). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using METTL3 Rabbit mAb (A26858) at a dilution of 1:1000.

ABclonal:Chromatin Immunoprecipitation - [KD Validated] METTL3 Rabbit mAb (A26858)

Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from MCF7, using 3 μg of [KD Validated] METTL3 Rabbit mAb (A26858) and Rabbit IgG isotype control (AC042). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.

Overview

Product name[KD Validated] METTL3 Rabbit mAb
Catalog No.A26858
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC66900
This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human METTL3 (NP_062826.2).
SequenceMSDTWSSIQAHKKQLDSLRERLQRRRKQDSGHLDLRNPEAALSPTFRSDSPVPTAPTSGGPKPSTASAVPELATDPELEKKLLHHLSDLALTLPTDAVSICLAISTPDAPATQDGVESLLQKFAAQELIEVKRGLLQDDAHPTLVTYADHSKLSAM
Gene ID
Swiss Prot
SynonymsM6A; IME4; Spo8; MT-A70; hMETTL3
Calculated MW64kDa
Observed MW75kDa/70KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:10000
  • IHC-P 1:200 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ChIP 5μg antibody for 10μg-15μg of Chromatin
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samples293T, Hep G2, Rat thymus, Mouse brain, NIH/3T3, HepG2
Cellular locationNucleus speckle.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - [KD Validated] METTL3 Rabbit mAb (A26858)}

    Western blot - [KD Validated] METTL3 Rabbit mAb (A26858)

    Western blot analysis of various lysates using [KD Validated] METTL3 Rabbit mAb (A26858) at 1:5000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - [KD Validated] METTL3 Rabbit mAb (A26858)}

    Western blot - [KD Validated] METTL3 Rabbit mAb (A26858)

    Western blot analysis of various lysates using [KD Validated] METTL3 Rabbit mAb (A26858) at 1:5000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - [KD Validated] METTL3 Rabbit mAb (A26858)}

    Immunohistochemistry - [KD Validated] METTL3 Rabbit mAb (A26858)

    Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using [KD Validated] METTL3 Rabbit mAb (A26858) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunoprecipitation - [KD Validated] METTL3 Rabbit mAb (A26858)}

    Immunoprecipitation - [KD Validated] METTL3 Rabbit mAb (A26858)

    Immunoprecipitation of METTL3 from 300 µg extracts of HepG2 cells was performed using 0.5 µg of [KD Validated] METTL3 Rabbit mAb (A26858). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using [KD Validated] METTL3 Rabbit mAb (A26858) at a dilution of 1 : 1000.
    ABclonal:Immunoprecipitation - [KD Validated] METTL3 Rabbit mAb (A26858)}

    Immunoprecipitation - [KD Validated] METTL3 Rabbit mAb (A26858)

    Immunoprecipitation of METTL3 from 300 µg extracts of HepG2 cells was performed using 0.5 µg of METTL3 Rabbit mAb (A26858). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using METTL3 Rabbit mAb (A26858) at a dilution of 1:1000.
    ABclonal:Chromatin Immunoprecipitation - [KD Validated] METTL3 Rabbit mAb (A26858)}

    Chromatin Immunoprecipitation - [KD Validated] METTL3 Rabbit mAb (A26858)

    Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from MCF7, using 3 μg of [KD Validated] METTL3 Rabbit mAb (A26858) and Rabbit IgG isotype control (AC042). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.

    * For research use only. Not for therapeutic or diagnostic purposes.

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