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[KD Validated] METTL3 Rabbit pAb (A8370)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] METTL3 Rabbit pAb (A8370)

Western blot analysis of lysates from wild type (WT) and METTL3 knockdown (KD) 293T cells using METTL3 Rabbit pAb (A8370) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of various lysates using METTL3 Rabbit pAb (A8370) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - [KD Validated] METTL3 Rabbit pAb (A8370)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using METTL3 Rabbit pAb (A8370) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse testis using METTL3 Rabbit pAb (A8370) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KD Validated] METTL3 Rabbit pAb (A8370)

Confocal immunofluorescence analysis of Hela cells using METTL3 Rabbit pAb (A8370) at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HepG2 cells using METTL3 Rabbit pAb (A8370) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-3 cells using METTL3 Rabbit pAb (A8370) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	[KD Validated] METTL3 Rabbit pAb
Catalog No.	A8370
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human METTL3 (NP_062826.2).
Sequence	MSDTWSSIQAHKKQLDSLRERLQRRRKQDSGHLDLRNPEAALSPTFRSDSPVPTAPTSGGPKPSTASAVPELATDPELEKKLLHHLSDLALTLPTDAVSICLAISTPDAPATQDGVESLLQKFAAQELIEVKRGLLQDDAHPTLVTYADHSKLSAM
Gene ID
Swiss Prot
Synonyms	M6A; IME4; Spo8; MT-A70; hMETTL3; METTL3
Calculated MW	64kDa
Observed MW	70kDa/75KDa/75kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	NIH/3T3, 293T, HeLa, Hep G2, Mouse testis
Cellular location	Nucleus speckle.
Customer validation	(Human breast cancer, HeLa) Co-IP(Homo sapiens) WB(Rattus norvegicus, Homo sapiens, Mus musculus, Capra hircus, Mus musculus) RIP(Homo sapiens, Mus musculus) PCR(Homo sapiens) IF(Homo sapiens, Mus musculus) IP(Mus musculus) IHC(Mus musculus) IP(Mus musculus) MeRIP(Mus musculus) IHC(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

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Research Area

[KD Validated] METTL3 Rabbit pAb images

Western blot - [KD Validated] METTL3 Rabbit pAb (A8370)

Immunohistochemistry - [KD Validated] METTL3 Rabbit pAb (A8370)

Immunofluorescence - [KD Validated] METTL3 Rabbit pAb (A8370)

Confocal immunofluorescence analysis of Hela cells using METTL3 Rabbit pAb (A8370) at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunofluorescence - [KD Validated] METTL3 Rabbit pAb (A8370)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A8370

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Product Protocols

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Molecular Cell

Application:

Co-IP

IF:

14.5

Species:

Homo sapiens

PMID:

29304330

Title:

N 6-Methyladenosines Modulate A-to-I RNA Editing

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

8.1

Species:

Rattus norvegicus

PMID:

31097692

Title:

Impaired autophagic degradation of lncRNA ARHGAP5-AS1 promotes chemoresistance in gastric cancer.

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Mol Cancer

Application:

RIP

IF:

37.3

Species:

Homo sapiens

PMID:

32838807

Title:

β-catenin represses miR455-3p to stimulate m6A modification of HSF1 mRNA and promote its translation in colorectal cancer

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Placenta

Application:

IF:

Species:

Homo sapiens

PMID:

33070036

Title:

Upregulation of METTL3 expression and m6A RNA methylation in placental trophoblasts in preeclampsia

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Cell Mol Gastroenterol Hepatol

Application:

IF:

7.1

Species:

Mus musculus

PMID:

33848642

Title:

Mettl14-Mediated m 6 A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Mol Cell Endocrinol

Application:

IF:

3.8

Species:

Rattus norvegicus

PMID:

34843901

Title:

The m6A mRNA demethylase FTO regulates GnRH secretion in Mn-induced precocious puberty

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Arterioscler Thromb Vasc Biol

Application:

IF:

7.4

Species:

Mus musculus

PMID:

34645279

Title:

Endothelial METTL3 (Methyltransferase-Like 3) Inhibits Fibrinolysis by Promoting PAI-1 (Plasminogen Activator Inhibitor-1) Expression Through Enhancing Jun Proto-Oncogene N6-Methyladenosine Modification

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Frontiers in cell and developmental biology

Application:

IF:

4.6

Species:

Homo sapiens

PMID:

35874829

Title:

The Maternal Microbiome Programs the m6A Epitranscriptome of the Mouse Fetal Brain and Intestine - PMC

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Archives of biochemistry and biophysics

Application:

PCR

IF:

3.8

Species:

Homo sapiens

PMID:

36027936

Title:

N6-methyladenosine-mediated upregulation of LINC00520 accelerates breast cancer progression via regulating miR-577/POSTN axis and downstream ILK/AKT …

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Oncogene

Application:

RIP

IF:

6.9

Species:

Homo sapiens

PMID:

35869392

Title:

PLAA suppresses ovarian cancer metastasis via METTL3-mediated m6A modification of TRPC3 mRNA - PMC

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Frontiers in oncology

Application:

IF:

3.5

Species:

Homo sapiens

PMID:

36313631

Title:

IGF2BP3 promotes progression of gallbladder carcinoma by stabilizing KLK5 mRNA in N6-methyladenosine-dependent binding - PMC

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Nature communications

Application:

IF:

16.6

Species:

Mus musculus

PMID:

36859428

Title:

Inhibition of ALKBH5 attenuates I/R-induced renal injury in male mice by promoting Ccl28 m6A modification and increasing Treg recruitment - PMC

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Journal of oncology

Application:

IF:

Species:

Homo sapiens

PMID:

36866236

Title:

The Comprehensive Analysis of N6-Methyadenosine Writer METTL3 and METTL14 in Gastric Cancer - PMC

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

International journal of biological sciences

Application:

IF:

8.2

Species:

Homo sapiens

PMID:

37056926

Title:

M6A-modified circRBM33 promotes prostate cancer progression via PDHA1-mediated mitochondrial respiration regulation and presents a potential target for ARSI therapy - PMC

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Translational cancer research

Application:

IF:

1.5

Species:

Homo sapiens

PMID:

37434681

Title:

In vitro study of piwi interaction RNA-31106 promoting breast carcinogenesis by regulating METTL3-mediated m6A RNA methylation - PMC

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

5.6

Species:

Capra hircus

PMID:

37762418

Title:

METTL3 Promotes the Differentiation of Goat Skeletal Muscle Satellite Cells by Regulating MEF2C mRNA Stability in a m6A-Dependent Manner - PMC

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Cells

Application:

WB, IF

IF:

7.7

Species:

Homo sapiens

PMID:

32033081

Title:

N6-Adenosine Methylation in RNA and a Reduced m3G/TMG Level in Non-Coding RNAs Appear at Microirradiation-Induced DNA Lesions

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Molecular therapy. Nucleic Acids

Application:

IF:

6.5

Species:

Homo sapiens, Mus musculus

PMID:

31896070

Title:

m6A Methylation of Precursor-miR-320/RUNX2 Controls Osteogenic Potential of Bone Marrow-Derived Mesenchymal Stem Cells

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Nature

Application:

WB, IF

IF:

50.5

Species:

Homo sapiens

PMID:

28297716

Title:

RNA m6A methylation regulates the ultraviolet-induced DNA damage response.

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Journal of Cancer

Application:

IF:

3.3

Species:

Homo sapiens

PMID:

37859814

Title:

Suppression of Wnt/-catenin Signaling in PDAC via METTL16-mediated N6-methyladenosine Modification of DVL2 - PMC

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

American journal of cancer research

Application:

IF:

3.6

Species:

Homo sapiens

PMID:

38187046

Title:

Estrogen receptor inhibits Caveolin 1 translation by promoting m6A-dependent miR199a-5p maturation to confer nab-paclitaxel resistance - PMC

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Nature communications

Application:

RIP

IF:

16.6

Species:

Homo sapiens, Mus musculus

PMID:

35273176

Title:

Fusobacterium nucleatum reduces METTL3-mediated m 6 A modification and contributes to colorectal cancer metastasis

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Advanced science

Application:

IF:

15.1

Species:

Homo sapiens

PMID:

38041494

Title:

mTORC1 Mediates Biphasic MechanoResponse to Orchestrate AdhesionDependent Cell Growth and Anoikis Resistance

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

EMBO reports

Application:

IF:

6.5

Species:

Mus musculus

PMID:

38182816

Title:

DDX5 inhibits inflammation by modulating m6A levels of TLR2/4 transcripts during bacterial infection

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Bone

Application:

WB, IHC, IF

IF:

3.5

Species:

Mus musculus

PMID:

34530171

Title:

Mettl3 inhibits the apoptosis and autophagy of chondrocytes in inflammation through mediating Bcl2 stability via Ythdf1-mediated m6A modification

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Breast cancer research : BCR

Application:

IF:

6.1

Species:

Homo sapiens

PMID:

36765397

Title:

METTL3 depletion contributes to tumour progression and drug resistance via N6 methyladenosine-dependent mechanism in HR+HER2—breast cancer - PMC

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Proc Natl Acad Sci U S A

Application:

WB, IHC, IF

IF:

9.4

Species:

Mus musculus

PMID:

33579825

Title:

METTL3-dependent N 6-methyladenosine RNA modification mediates the atherogenic inflammatory cascades in vascular endothelium

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

WB, IF

IF:

5.6

Species:

Mus musculus

PMID:

33143367

Title:

The distinct function and localization of METTL3/METTL14 and METTL16 enzymes in cardiomyocytes

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Bone

Application:

WB, IHC, IF

IF:

3.5

Species:

Mus musculus

PMID:

35981698

Title:

Mettl3 regulates hypertrophic differentiation of chondrocytes through modulating Dmp1 mRNA via Ythdf1-mediated m6A modification

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Molecular cell

Application:

WB, IHC, IP

IF:

14.5

Species:

Mus musculus

PMID:

35320754

Title:

Lactylation-driven METTL3-mediated RNA m 6 A modification promotes immunosuppression of tumor-infiltrating myeloid cells

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

The Science of the total environment

Application:

MeRIP

IF:

8.2

Species:

Mus musculus

PMID:

36423848

Title:

m6A contributes to a pro-survival state in GC-2 cells by facilitating DNA damage repair: Novel perspectives on the mechanism underlying DEHP genotoxicity in male

References for [KD Validated] METTL3 Rabbit pAb (A8370)

Product:

[KD Validated] METTL3 Rabbit pAb

Journal:

Cell chemical biology

Application:

IHC

IF:

8.6

Species:

Homo sapiens,Mus musculus

PMID: