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[KD Validated] NUR77 Rabbit mAb (A24016)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - [KD Validated] NUR77 Rabbit mAb (A24016)

Western blot analysis of lysates from PC-3 cells, using [KD Validated] NUR77 Rabbit mAb (A24016) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KD Validated] NUR77 Rabbit mAb (A24016)

Western blot analysis of lysates from wild type(WT) and NUR77 knockdown (KD) HeLa cells, using [KD Validated] NUR77 Rabbit mAb (A24016) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product name[KD Validated] NUR77 Rabbit mAb
Catalog No.A24016
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC58221
This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 250-350 of human NUR77 (NP_002126.2)
SequencePSLAQSPLKLFPSQATHQLGEGESYSMPTAFPGLAPTSPHLEGSGILDTPVTSTKARSGAPGGSEGRCAVCGDNASCQHYGVRTCEGCKGFFKRTVQKNAK
Gene ID
Swiss Prot
SynonymsHMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77; [KD Validated] NUR77
Calculated MW64kDa
Observed MW64kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, PC-3
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KD Validated] NUR77 Rabbit mAb (A24016)}

Western blot - [KD Validated] NUR77 Rabbit mAb (A24016)

Western blot analysis of lysates from PC-3 cells, using [KD Validated] NUR77 Rabbit mAb (A24016) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KD Validated] NUR77 Rabbit mAb (A24016)}

Western blot - [KD Validated] NUR77 Rabbit mAb (A24016)

Western blot analysis of lysates from wild type(WT) and NUR77 knockdown (KD) HeLa cells, using [KD Validated] NUR77 Rabbit mAb (A24016) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)