Western blot - [KD Validated] PTBP1 Rabbit mAb (A25184)
Western blot analysis of lysates from wild type (WT) and PTBP1 knockdown (KD) HeLa cells using [KD Validated] PTBP1 Rabbit mAb (A25184) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Western blot - [KD Validated] PTBP1 Rabbit mAb (A25184)
Western blot analysis of lysates from Mouse lung using [KD Validated] PTBP1 Rabbit mAb (A25184) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Immunohistochemistry - [KD Validated] PTBP1 Rabbit mAb (A25184)
Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using [KD Validated] PTBP1 Rabbit mAb (A25184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KD Validated] PTBP1 Rabbit mAb (A25184)
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KD Validated] PTBP1 Rabbit mAb (A25184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KD Validated] PTBP1 Rabbit mAb (A25184)
Immunohistochemistry analysis of paraffin-embedded Mouse intestine tissue using [KD Validated] PTBP1 Rabbit mAb (A25184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KD Validated] PTBP1 Rabbit mAb (A25184)
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] PTBP1 Rabbit mAb (A25184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KD Validated] PTBP1 Rabbit mAb (A25184)
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KD Validated] PTBP1 Rabbit mAb (A25184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KD Validated] PTBP1 Rabbit mAb (A25184)
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] PTBP1 Rabbit mAb (A25184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KD Validated] PTBP1 Rabbit mAb (A25184)
Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using [KD Validated] PTBP1 Rabbit mAb (A25184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KD Validated] PTBP1 Rabbit mAb (A25184)
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KD Validated] PTBP1 Rabbit mAb (A25184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KD Validated] PTBP1 Rabbit mAb (A25184)
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using [KD Validated] PTBP1 Rabbit mAb (A25184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence - [KD Validated] PTBP1 Rabbit mAb (A25184)
Confocal imaging of HeLa cells using [KD Validated] PTBP1 Rabbit mAb (A25184, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunofluorescence - [KD Validated] PTBP1 Rabbit mAb (A25184)
Confocal imaging of NIH/3T3 cells using [KD Validated] PTBP1 Rabbit mAb (A25184, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunoprecipitation - [KD Validated] PTBP1 Rabbit mAb (A25184)
Immunoprecipitation of PTBP1 in 500 µg extracts from Mouse lung using 2 µg [KD Validated] PTBP1 Rabbit mAb (A25184). Western blot analysis was performed using [KD Validated] PTBP1 Rabbit mAb (A25184) at 1:1000 dilution.