제품 > 항체 > 다중클론항체(pAb)

PTBP1 Rabbit pAb (A6107)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HeLa cells, using PTBP1 Rabbit pAb (A6107) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of various lysates, using PTBP1 Rabbit pAb (A6107) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - PTBP1 Rabbit pAb (A6107)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using PTBP1 Rabbit pAb (A6107) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - PTBP1 Rabbit pAb (A6107)

Immunofluorescence analysis of U2OS cells using PTBP1 Rabbit pAb (A6107) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	PTBP1 Rabbit pAb
Catalog No.	A6107
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PTBP1 (NP_114368.1).
Sequence	MDGIVPDIAVGTKRGSDELFSTCVTNGPFIMSSNSASAANGNDSKKFKGDSRSAGVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIE
Gene ID
Swiss Prot
Synonyms	PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I; PTBP1
Calculated MW	60kDa
Observed MW	59kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:100 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa, NIH/3T3
Cellular location	Nucleus.
Customer validation	WB(Homo sapiens, Mus musculus) IF(Mus musculus) IHC(Mus musculus) IF(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

연구후성유전학 & 핵 신호전달RNA 결합

PTBP1 Rabbit pAb images

Western blot - PTBP1 Rabbit pAb (A6107)

Immunohistochemistry - PTBP1 Rabbit pAb (A6107)

Immunofluorescence - PTBP1 Rabbit pAb (A6107)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for PTBP1 Rabbit pAb (A6107)

Product:

PTBP1 Rabbit pAb

Journal:

Experimental Cell Research

Application:

IF:

3.3

Species:

Homo sapiens

PMID:

31837293

Title:

Knock-down of circular RNA H19 induces human adipose-derived stem cells adipogenic differentiation via a mechanism involving the polypyrimidine tract-binding protein 1

References for PTBP1 Rabbit pAb (A6107)

Product:

PTBP1 Rabbit pAb

Journal:

Nat Neurosci

Application:

IF:

21.2

Species:

Mus musculus

PMID:

34083786

Title:

Therapeutically viable generation of neurons with antisense oligonucleotide suppression of PTB

References for PTBP1 Rabbit pAb (A6107)

Product:

PTBP1 Rabbit pAb

Journal:

Nat Chem Biol

Application:

IF:

12.9

Species:

Mus musculus

PMID:

34916619

Title:

Phase separation of RNA-binding protein promotes polymerase binding and transcription

References for PTBP1 Rabbit pAb (A6107)

Product:

PTBP1 Rabbit pAb

Journal:

Cell

Application:

IF:

45.5

Species:

Mus musculus

PMID:

34582787

Title:

Revisiting astrocyte to neuron conversion with lineage tracing in vivo

References for PTBP1 Rabbit pAb (A6107)

Product:

PTBP1 Rabbit pAb

Journal:

Frontiers in aging neuroscience

Application:

IF:

4.1

Species:

Mus musculus

PMID:

37181622

Title:

Polypyrimidine tract binding protein knockdown reverses depression-like behaviors and cognition impairment in mice with lesioned cholinergic neurons - PMC

References for PTBP1 Rabbit pAb (A6107)

Product:

PTBP1 Rabbit pAb

Journal:

Experimental cell research

Application:

IF:

3.3

Species:

Homo sapiens

PMID:

35405117