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[KD Validated] XPNPEP3 Rabbit mAb (A26950)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] XPNPEP3 Rabbit mAb (A26950)

Western blot analysis of lysates from wild type (WT) and XPNPEP3 knockdown (KD) 293T cells using [KD Validated] XPNPEP3 Rabbit mAb (A26950) at 1:13000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KD Validated] XPNPEP3 Rabbit mAb (A26950)

Western blot analysis of various lysates using [KD Validated] XPNPEP3 Rabbit mAb (A26950) at 1:13000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KD Validated] XPNPEP3 Rabbit mAb (A26950)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] XPNPEP3 Rabbit mAb (A26950) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] XPNPEP3 Rabbit mAb (A26950)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] XPNPEP3 Rabbit mAb (A26950) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] XPNPEP3 Rabbit mAb (A26950)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] XPNPEP3 Rabbit mAb (A26950) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product name[KD Validated] XPNPEP3 Rabbit mAb
Catalog No.A26950
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC70227
The protein encoded by this gene belongs to the family of X-pro-aminopeptidases that utilize a metal cofactor, and remove the N-terminal amino acid from peptides with a proline residue in the penultimate position. This protein has been shown to localize to the mitochondria of renal cells, and have a role in ciliary function. Mutations in this gene are associated with nephronophthisis-like nephropathy-1. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene, however, expression of some of these isoforms in vivo is not known.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 32-230 of human XPNPEP3 (NP_071381.1).
SequenceSLQPVPERRIPNRYLGQPSPFTHPHLLRPGEVTPGLSQVEYALRRHKLMSLIQKEAQGQSGTDQTVVVLSNPTYYMSNDIPYTFHQDNNFLYLCGFQEPDSILVLQSLPGKQLPSHKAILFVPRRDPSRELWDGPRSGTDGAIALTGVDEAYTLEEFQHLLPKMKAETNMVWYDWMRPSHAQLHSDYMQPLTEAKAKSK
Gene ID
Swiss Prot
SynonymsAPP3; ICP55; NPHPL1
Calculated MW57kDa
Observed MW57kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:13000 - 1:78000
  • IHC-P 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesA-431, Mouse kidney, 293T
Cellular locationMitochondrion.

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KD Validated] XPNPEP3 Rabbit mAb (A26950)}

Western blot - [KD Validated] XPNPEP3 Rabbit mAb (A26950)

Western blot analysis of lysates from wild type (WT) and XPNPEP3 knockdown (KD) 293T cells using [KD Validated] XPNPEP3 Rabbit mAb (A26950) at 1:13000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KD Validated] XPNPEP3 Rabbit mAb (A26950)}

Western blot - [KD Validated] XPNPEP3 Rabbit mAb (A26950)

Western blot analysis of various lysates using [KD Validated] XPNPEP3 Rabbit mAb (A26950) at 1:13000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KD Validated] XPNPEP3 Rabbit mAb (A26950)}

Immunohistochemistry - [KD Validated] XPNPEP3 Rabbit mAb (A26950)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] XPNPEP3 Rabbit mAb (A26950) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] XPNPEP3 Rabbit mAb (A26950)}

Immunohistochemistry - [KD Validated] XPNPEP3 Rabbit mAb (A26950)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] XPNPEP3 Rabbit mAb (A26950) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] XPNPEP3 Rabbit mAb (A26950)}

Immunohistochemistry - [KD Validated] XPNPEP3 Rabbit mAb (A26950)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] XPNPEP3 Rabbit mAb (A26950) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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