제품 > 항체 > 다중클론항체(pAb)

[KD Validated] YTHDC2 Rabbit pAb (A24219)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using [KD Validated] YTHDC2 Rabbit pAb (A24219) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 30s.

Western blot analysis of lysates from wild type (WT) and YTHDC2 knockdown (KD) HeLa cells using [KD Validated] YTHDC2 Rabbit pAb (A24219) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 30s.

ABclonal:Immunofluorescence - [KD Validated] YTHDC2 Rabbit pAb (A24219)

Immunofluorescence analysis of paraffin-embedded Mouse testis using [KD Validated] YTHDC2 Rabbit pAb(A24219) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of paraffin-embedded Rat testis using [KD Validated] YTHDC2 Rabbit pAb(A24219) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KD Validated] YTHDC2 Rabbit pAb (A24219)

Immunoprecipitation of YTHDC2 in 200 µg extracts from 293T cells using 0.5 µg [KD Validated] YTHDC2 Rabbit pAb (A24219). Western blot analysis was performed using [KD Validated] YTHDC2 Rabbit pAb (A24219) at 1:1000 dilution.

Overview

Product name	[KD Validated] YTHDC2 Rabbit pAb
Catalog No.	A24219
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein binds to N6-methyladenosine, a common modified RNA nucleotide that is enriched in the stop codons and 3' UTRs of eukaryotic messenger RNAs. Binding of proteins to this modified nucleotide may regulate mRNA translation and stability. This gene may be associated with susceptibility to pancreatic cancer in human patients, and knockdown of this gene resulted in reduced proliferation in a human liver cancer cell line.

Immunogen information

Immunogen	Recombinant Protein corresponding to a sequence within amino acids 1286-1430 of human YTHDC2(NP_073739.3).
Sequence	MPVRYFIMKSSNLRNLEISQQKGIWSTTPSNERKLNRAFWESSIVYLVFSVQGSGHFQGFSRMSSEIGREKSQDWGSAGLGGVFKVEWIRKESLPFQFAHHLLNPWNDNKKVQISRDGQELEPLVGEQLLQLWERLPLGEKNTTD
Gene ID
Swiss Prot
Synonyms	CAHL; hYTHDC2; YTHDC2
Calculated MW	160kDa
Observed MW	170kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	293T, NIH/3T3, PC-12, HeLa
Cellular location	endoplasmic reticulum, nucleus, perinuclear region of cytoplasm