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[KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Western blot analysis of various lysates using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189) at 1:13000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Western blot analysis of lysates from wild type (WT) and hnRNP A2B1 knockdown (KD) HeLa cells using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189) at 1:13000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189) at a dilution of 1:7000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189) at a dilution of 1:7000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189) at a dilution of 1:7000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189) at a dilution of 1:7000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189) at a dilution of 1:7000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Confocal imaging of HeLa cells using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Confocal imaging of A-431 cells using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Confocal imaging of 293T cells using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Confocal imaging of NIH/3T3 cells using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product name[KD Validated] hnRNP A2B1 Rabbit mAb
Catalog No.A27189
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC72144
This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 151-250 of human HNRNPA2B1 (NP_112533.1).
SequenceKKRGFGFVTFDDHDPVDKIVLQKYHTINGHNAEVRKALSRQEMQEVQSSRSGRGGNFGFGDSRGGGGNFGPGPGSNFRGGSDGYGSGRGFGDGYNGYGGG
Gene ID
Swiss Prot
SynonymsRNPA2; HNRPA2; HNRPB1; SNRPB1; HNRNPA2; HNRNPB1; IBMPFD2; HNRPA2B1
Calculated MW37kDa
Observed MW34-38kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:13000 - 1:78000
  • IHC-P 1:3000 - 1:30000
  • IF/ICC 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-549, NIH/3T3, RAW 264.7, PC-12, HeLa
Cellular locationCytoplasm, Nucleus, nucleoplasm.

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)}

Western blot - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Western blot analysis of various lysates using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189) at 1:13000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)}

Western blot - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Western blot analysis of lysates from wild type (WT) and hnRNP A2B1 knockdown (KD) HeLa cells using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189) at 1:13000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)}

Immunohistochemistry - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189) at a dilution of 1:7000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)}

Immunohistochemistry - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189) at a dilution of 1:7000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)}

Immunohistochemistry - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189) at a dilution of 1:7000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)}

Immunohistochemistry - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189) at a dilution of 1:7000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)}

Immunohistochemistry - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189) at a dilution of 1:7000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)}

Immunofluorescence - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Confocal imaging of HeLa cells using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)}

Immunofluorescence - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Confocal imaging of A-431 cells using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)}

Immunofluorescence - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Confocal imaging of 293T cells using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)}

Immunofluorescence - [KD Validated] hnRNP A2B1 Rabbit mAb (A27189)

Confocal imaging of NIH/3T3 cells using [KD Validated] hnRNP A2B1 Rabbit mAb (A27189, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

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