제품 > 항체 > 다중클론항체(pAb)

KIF20A Rabbit pAb (A15377)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KIF20A Rabbit pAb (A15377)

Western blot analysis of various lysates using KIF20A Rabbit pAb (A15377) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

Overview

Product nameKIF20A Rabbit pAb
Catalog No.A15377
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables protein kinase binding activity. Involved in microtubule bundle formation; midbody abscission; and regulation of cytokinesis. Located in several cellular components, including cleavage furrow; intercellular bridge; and midbody. Implicated in restrictive cardiomyopathy.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 591-890 of human KIF20A (NP_005724.1).
SequenceEICNEMVEQMQQREQWCSEHLDTQKELLEEMYEEKLNILKESLTSFYQEEIQERDEKIEELEALLQEARQQSVAHQQSGSELALRRSQRLAASASTQQLQEVKAKLQQCKAELNSTTEELHKYQKMLEPPPSAKPFTIDVDKKLEEGQKNIRLLRTELQKLGESLQSAERACCHSTGAGKLRQALTTCDDILIKQDQTLAELQNNMVLVKLDLRKKAACIAEQYHTVLKLQGQVSAKKRLGTNQENQQPNQQPPGKKPFLRNLLPRTPTCQSSTDCSPYARILRSRRSPLLKSGPFGKKY
Gene ID
Swiss Prot
SynonymsRCM6; MKLP2; RAB6KIFL; KIF20A
Calculated MW100kDa
Observed MW105kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, A-549, Mouse spleen, Mouse testis, Mouse heart, Mouse lung, Mouse brain, Rat spleen
Cellular locationCytoplasm, Golgi apparatus, cytoskeleton, spindle.
Customer validation

WB(Mus musculus)

Documents

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ABclonal:Western blot - KIF20A Rabbit pAb (A15377)}

Western blot - KIF20A Rabbit pAb (A15377)

Western blot analysis of various lysates using KIF20A Rabbit pAb (A15377) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

* For research use only. Not for therapeutic or diagnostic purposes.

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