제품 > 항체 > 다중클론항체(pAb)

KLB Rabbit pAb (A15629)

Reviews (2)Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KLB Rabbit pAb (A15629)

Western blot analysis of various lysates using KLB Rabbit pAb (A15629) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Review
ABclonal: review for KLB Rabbit pAb(A15629)
Review
ABclonal: review for KLB Rabbit pAb(A15629)

Overview

Product nameKLB Rabbit pAb
Catalog No.A15629
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables fibroblast growth factor binding activity and fibroblast growth factor receptor binding activity. Predicted to be involved in fibroblast growth factor receptor signaling pathway. Predicted to act upstream of or within positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway and positive regulation of cell population proliferation. Predicted to be located in plasma membrane. Predicted to be active in endoplasmic reticulum.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 720-990 of human KLB (NP_783864.1).
SequenceALAWRLYDRQFRPSQRGAVSLSLHADWAEPANPYADSHWRAAERFLQFEIAWFAEPLFKTGDYPAAMREYIASKHRRGLSSSALPRLTEAERRLLKGTVDFCALNHFTTRFVMHEQLAGSRYDSDRDIQFLQDITRLSSPTRLAVIPWGVRKLLRWVRRNYGDMDIYITASGIDDQALEDDRLRKYYLGKYLQEVLKAYLIDKVRIKGYYAFKLAEEKSKPRFGFFTSDFKAKSSIQFYNKVISSRGFPFENSSSRCSQTQENTECTVCLF
Gene ID
Swiss Prot
SynonymsBKL; KLB
Calculated MW120kDa
Observed MW120kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesLO2, SGC-7901, rat liver
Cellular locationplasma membrane
Customer validation

(HeLa cells)

WB(Homo sapiens, Rattus norvegicus, Mus musculus)

IHC(Rattus norvegicus)

IF(Rattus norvegicus)

ABclonal:Western blot - KLB Rabbit pAb (A15629)}

Western blot - KLB Rabbit pAb (A15629)

Western blot analysis of various lysates using KLB Rabbit pAb (A15629) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

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재조합 단백질 (1)

Secondary Antibodies (26)