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KLHDC3 Rabbit pAb (A13741)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KLHDC3 Rabbit pAb (A13741)

Western blot analysis of various lysates using KLHDC3 Rabbit pAb (A13741) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - KLHDC3 Rabbit pAb (A13741)

Immunofluorescence analysis of HeLa cells using KLHDC3 Rabbit pAb (A13741) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - KLHDC3 Rabbit pAb (A13741)

Immunofluorescence analysis of PC-12 cells using KLHDC3 Rabbit pAb (A13741) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameKLHDC3 Rabbit pAb
Catalog No.A13741
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene contains six repeated kelch motifs that are structurally similar to recombination activating gene 2, a protein involved in the activation of the V(D)J recombination. In mouse, this gene is found to be expressed specifically in testis. Its expression in pachytene spermatocytes is localized to cytoplasma and meiotic chromatin, suggesting that this gene may be involved in meiotic recombination.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-382 of human KLHDC3 (NP_476502.1).
SequenceMLRWTVHLEGGPRRVNHAAVAVGHRVYSFGGYCSGEDYETLRQIDVHIFNAVSLRWTKLPPVKSAIRGQAPVVPYMRYGHSTVLIDDTVLLWGGRNDTEGACNVLYAFDVNTHKWFTPRVSGTVPGARDGHSACVLGKIMYIFGGYEQQADCFSNDIHKLDTSTMTWTLICTKGSPARWRDFHSATMLGSHMYVFGGRADRFGPFHSNNEIYCNRIRVFDTRTEAWLDCPPTPVLPEGRRSHSAFGYNGELYIFGGYNARLNRHFHDLWKFNPVSFTWKKIEPKGKGPCPRRRQCCCIVGDKIVLFGGTSPSPEEGLGDEFDLIDHSDLHILDFSPSLKTLCKLAVIQYNLDQSCLPHDIRWELNAMTTNSNISRPIVSSHG
Gene ID
Swiss Prot
SynonymsPEAS; dJ20C7.3; KLHDC3
Calculated MW43kDa
Observed MW43kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesNCI-H460, DU145, HepG2
Cellular locationCytoplasm.
Customer validation

WB(Homo sapiens)

IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - KLHDC3 Rabbit pAb (A13741)}

    Western blot - KLHDC3 Rabbit pAb (A13741)

    Western blot analysis of various lysates using KLHDC3 Rabbit pAb (A13741) at 1:3000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - KLHDC3 Rabbit pAb (A13741)}

    Immunofluorescence - KLHDC3 Rabbit pAb (A13741)

    Immunofluorescence analysis of HeLa cells using KLHDC3 Rabbit pAb (A13741) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - KLHDC3 Rabbit pAb (A13741)}

    Immunofluorescence - KLHDC3 Rabbit pAb (A13741)

    Immunofluorescence analysis of PC-12 cells using KLHDC3 Rabbit pAb (A13741) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A13741? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)