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[KO Validated] CDK2 Rabbit mAb (A0094)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using CDK2 Rabbit mAb (A0094) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of lysates from wild type(WT) and CDK2 knockout (KO) 293T cells, using [KO Validated] CDK2 Rabbit mAb (A0094) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Review

Overview

Product name	[KO Validated] CDK2 Rabbit mAb
Catalog No.	A0094
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0222

Background

This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 199-298 of human CDK2 (P24941).
Sequence	RRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL
Gene ID
Swiss Prot
Synonyms	CDKN2; p33(CDK2); K2
Calculated MW	34kDa
Observed MW	33kDa/38kDa(mouse rat)

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:6000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa, 293T, Jurkat, Mouse spleen, Rat testis
Cellular location	Cajal body, Cytoplasm, Endosome, Nucleus, centrosome, cytoskeleton, microtubule organizing center.
Customer validation	(HeLa 、PC-3、Jurkat 、HT29 、393T 、 RAMOS 、NCI-N87 、U2-OS , Hela PC-3 Jurkat HT-29 293T RAMOS NCI-N87 U2-OS FUOV-1) WB(Homo sapiens, Sus scrofa, Mus musculus, Oryctolagus cuniculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] CDK2 Rabbit mAb images

Western blot - [KO Validated] CDK2 Rabbit mAb (A0094)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for [KO Validated] CDK2 Rabbit mAb (A0094)

Product:

[KO Validated] CDK2 Rabbit mAb

Journal:

J Cell Mol Med

Application:

IF:

5.31

Species:

Homo sapiens

PMID:

32519437

Title:

Low levels of AMPK promote epithelial‐mesenchymal transition in lung cancer primarily through HDAC4‐and HDAC5‐mediated metabolic reprogramming

References for [KO Validated] CDK2 Rabbit mAb (A0094)

Product:

[KO Validated] CDK2 Rabbit mAb

Journal:

Biomed Pharmacother

Application:

IF:

6.529

Species:

Homo sapiens

PMID:

34148735

Title:

Isothiocyanate Iberin inhibits cell proliferation and induces cell apoptosis in the progression of ovarian cancer by mediating ROS accumulation and GPX1 expression

References for [KO Validated] CDK2 Rabbit mAb (A0094)

Product:

[KO Validated] CDK2 Rabbit mAb

Journal:

Nature communications

Application:

IF:

17.69

Species:

Homo sapiens

PMID:

35987950

Title:

Spatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labeling - PMC

References for [KO Validated] CDK2 Rabbit mAb (A0094)

Product:

[KO Validated] CDK2 Rabbit mAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Homo sapiens

PMID:

36768821

Title:

Short-Term Starvation Weakens the Efficacy of Cell Cycle Specific Chemotherapy Drugs through G1 Arrest - PMC

References for [KO Validated] CDK2 Rabbit mAb (A0094)

Product:

[KO Validated] CDK2 Rabbit mAb

Journal:

Oncology letters

Application:

IF:

3.11

Species:

Homo sapiens

PMID:

36909367

Title:

LncRNA SPRY4-IT1 is upregulated and promotes the proliferation of prostate cancer cells under hypoxia in vitro - PMC

References for [KO Validated] CDK2 Rabbit mAb (A0094)

Product:

[KO Validated] CDK2 Rabbit mAb

Journal:

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Application:

IF:

5.83

Species:

Homo sapiens

PMID:

36961375

Title:

Discovery of novel CDK2 inhibitors using multistage virtual screening and in vitro melanoma cell lines

References for [KO Validated] CDK2 Rabbit mAb (A0094)

Product:

[KO Validated] CDK2 Rabbit mAb

Journal:

Toxics

Application:

IF:

4.47

Species:

Sus scrofa

PMID:

37999575

Title:

N-Acetyl-L-Cysteine Ameliorates BPAF-Induced Porcine Sertoli Cell Apoptosis and Cell Cycle Arrest via Inhibiting the ROS Level - PMC

References for [KO Validated] CDK2 Rabbit mAb (A0094)

Product:

[KO Validated] CDK2 Rabbit mAb

Journal:

Cell death discovery

Application:

IF:

7.1

Species:

Homo sapiens

PMID:

38086844

Title:

Cepharanthine, a regulator of keap1-Nrf2, inhibits gastric cancer growth through oxidative stress and energy metabolism pathway - PMC

References for [KO Validated] CDK2 Rabbit mAb (A0094)

Product:

[KO Validated] CDK2 Rabbit mAb

Journal:

BMC cancer

Application:

IF:

4.63

Species:

Homo sapiens

PMID:

38167018

Title:

RACGAP1 promotes the progression and poor prognosis of lung adenocarcinoma through its effects on the cell cycle and tumor stemness - PMC

References for [KO Validated] CDK2 Rabbit mAb (A0094)

Product:

[KO Validated] CDK2 Rabbit mAb

Journal:

Journal of environmental sciences (China)

Application:

IF:

6.79

Species:

Mus musculus

PMID:

38135418

Title:

Decabromodiphenyl ether induces the chromosome association disorders of spermatocytes and deformation failures of spermatids in mice

References for [KO Validated] CDK2 Rabbit mAb (A0094)

Product:

[KO Validated] CDK2 Rabbit mAb

Journal:

Cells

Application:

IF:

7.66

Species:

Homo sapiens

PMID:

36429034

Title:

LTB4R Promotes the Occurrence and Progression of Clear Cell Renal Cell Carcinoma (ccRCC) by Regulating the AKT/mTOR Signaling Pathway - PMC

References for [KO Validated] CDK2 Rabbit mAb (A0094)

Product:

[KO Validated] CDK2 Rabbit mAb

Journal:

Apoptosis : an international journal on programmed cell death

Application:

IF:

7.2

Species:

Homo sapiens

PMID:

38553613

Title:

Deubiquitinating enzyme OTUD4 stabilizes RBM47 to induce ATF3 transcription: a novel mechanism underlying the restrained malignant properties of ccRCC cells

References for [KO Validated] CDK2 Rabbit mAb (A0094)

Product:

[KO Validated] CDK2 Rabbit mAb

Journal:

American journal of cancer research

Application:

IF:

5.3

Species:

Homo sapiens

PMID:

38590396