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Phospho-CDK2-Y15 Rabbit mAb (AP1005)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CDK2-Y15 Rabbit mAb (AP1005) at 1:1000 dilution. NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37℃ for 20 hours or treated by Nocodazole (100 ng/mL) at 37℃ for 24 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Overview

Product name	Phospho-CDK2-Y15 Rabbit mAb
Catalog No.	AP1005
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC1550

Background

This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y15 of human CDK2 (NP_001789.2).
Sequence	GTYGV
Gene ID
Swiss Prot
Synonyms	CDKN2; p33(CDK2); Phospho-CDK2-Y15
Calculated MW	34kDa
Observed MW	34kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	NIH/3T3 treated by Hydroxyurea, NIH/3T3 treated by Nocodazole
Cellular location	Cajal body, Cytoplasm, Endosome, Nucleus, centrosome, cytoskeleton, microtubule organizing center.
Customer validation	WB(Other) IF(Other)