제품 > 항체 > 단일클론항체(mAb)

[KO Validated] CDK4 Rabbit mAb (A11136)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat, Monkey

Western blot analysis of various lysates, using CDK4 Rabbit mAb (A11136) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of lysates from wild type(WT) and CDK4 Rabbit mAb knockout (KO) HeLa(KO) cells, using CDK4 Rabbit mAb (A11136) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] CDK4 Rabbit mAb (A11136)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KO Validated] CDK4 Rabbit mAb (A11136) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KO Validated] CDK4 Rabbit mAb (A11136) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using [KO Validated] CDK4 Rabbit mAb (A11136) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human prostate tissue using [KO Validated] CDK4 Rabbit mAb (A11136) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	[KO Validated] CDK4 Rabbit mAb
Catalog No.	A11136
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC51004

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 204-303 of human CDK4 (NP_000066.1).
Sequence	FAEMFRRKPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVSLPRGAFPPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKDEGNPE
Gene ID
Swiss Prot
Synonyms	CMM3; PSK-J3; K4
Calculated MW	34kDa
Observed MW	34kDa

Applications

Reactivity	Human, Mouse, Rat, Monkey
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:6000 IHC-P 1:200 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HeLa, COS-7, Mouse lung, Rat lung
Cellular location	Cytoplasm, Membrane, Nucleus.
Customer validation	WB(Homo sapiens, Mus musculus, Sus scrofa) IHC(Homo sapiens) WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] CDK4 Rabbit mAb images

Western blot - [KO Validated] CDK4 Rabbit mAb (A11136)

Immunohistochemistry - [KO Validated] CDK4 Rabbit mAb (A11136)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] CDK4 Rabbit mAb (A11136)

Product:

[KO Validated] CDK4 Rabbit mAb

Journal:

Evidence-based complementary and alternative medicine : eCAM

Application:

IF:

2.65

Species:

Homo sapiens

PMID:

35911149

Title:

Arisaema heterophyllum Blume Monomer Stigmasterol Targets PPARγ and Inhibits the Viability and Tumorigenicity of Lung Adenocarcinoma Cells NCI-H1975 - PMC

References for [KO Validated] CDK4 Rabbit mAb (A11136)

Product:

[KO Validated] CDK4 Rabbit mAb

Journal:

Disease markers

Application:

IF:

3.46

Species:

Homo sapiens

PMID:

35769815

Title:

Inhibition of DEK Enhances Doxorubicin-Induced Apoptosis and Cell Cycle Arrest in T-Cell Acute Lymphoblastic Leukemia Cells - PMC

References for [KO Validated] CDK4 Rabbit mAb (A11136)

Product:

[KO Validated] CDK4 Rabbit mAb

Journal:

Journal of hazardous materials

Application:

IF:

14.22

Species:

Mus musculus

PMID:

36493639

Title:

Prenatal and Postnatal Exposure to Polystyrene Microplastics Induces Testis Developmental Disorder and Affects Male Fertility in Mice

References for [KO Validated] CDK4 Rabbit mAb (A11136)

Product:

[KO Validated] CDK4 Rabbit mAb

Journal:

Cell death discovery

Application:

IF:

7.1

Species:

Mus musculus

PMID:

36934091

Title:

JMJD1C promotes smooth muscle cell proliferation by activating glycolysis in pulmonary arterial hypertension - PMC

References for [KO Validated] CDK4 Rabbit mAb (A11136)

Product:

[KO Validated] CDK4 Rabbit mAb

Journal:

Proteome science

Application:

IF:

2.88

Species:

Mus musculus

PMID:

37821903

Title:

Proteomic analysis of neonatal mouse hearts shows PKA functions as a cardiomyocyte replication regulator - PMC

References for [KO Validated] CDK4 Rabbit mAb (A11136)

Product:

[KO Validated] CDK4 Rabbit mAb

Journal:

European journal of pharmacology

Application:

IF:

5.19

Species:

Homo sapiens

PMID:

37460054

Title:

Network pharmacology analysis of icariside II against bladder cancer

References for [KO Validated] CDK4 Rabbit mAb (A11136)

Product:

[KO Validated] CDK4 Rabbit mAb

Journal:

Toxics

Application:

IF:

4.47

Species:

Sus scrofa

PMID:

37999575

Title:

N-Acetyl-L-Cysteine Ameliorates BPAF-Induced Porcine Sertoli Cell Apoptosis and Cell Cycle Arrest via Inhibiting the ROS Level - PMC

References for [KO Validated] CDK4 Rabbit mAb (A11136)

Product:

[KO Validated] CDK4 Rabbit mAb

Journal:

Cancer cell international

Application:

IF:

6.429

Species:

Homo sapiens

PMID:

35264157

Title:

Novel sequential therapy with metformin enhances the effects of cisplatin in testicular germ cell tumours via YAP1 signalling

References for [KO Validated] CDK4 Rabbit mAb (A11136)

Product:

[KO Validated] CDK4 Rabbit mAb

Journal:

Cellular signalling

Application:

IF:

4.85

Species:

Homo sapiens

PMID:

38040402

Title:

Deubiquitinating enzyme PSMD7 promotes bladder cancer development: Involvement of RAB1A stabilization

References for [KO Validated] CDK4 Rabbit mAb (A11136)

Product:

[KO Validated] CDK4 Rabbit mAb

Journal:

Journal of experimental & clinical cancer research : CR

Application:

IF:

Species:

Homo sapiens

PMID:

37303063

Title:

B4GALT1 promotes immune escape by regulating the expression of PD-L1 at multiple levels in lung adenocarcinoma - PMC

References for [KO Validated] CDK4 Rabbit mAb (A11136)

Product:

[KO Validated] CDK4 Rabbit mAb

Journal:

Nature communications

Application:

IF:

16.6

Species:

Mus musculus

PMID:

38424044

Title:

MYC induces CDK4/6 inhibitors resistance by promoting pRB1 degradation - PMC

References for [KO Validated] CDK4 Rabbit mAb (A11136)

Product:

[KO Validated] CDK4 Rabbit mAb

Journal:

Cells

Application:

IF:

7.66

Species:

Homo sapiens

PMID:

36429034

Title:

LTB4R Promotes the Occurrence and Progression of Clear Cell Renal Cell Carcinoma (ccRCC) by Regulating the AKT/mTOR Signaling Pathway - PMC

References for [KO Validated] CDK4 Rabbit mAb (A11136)

Product:

[KO Validated] CDK4 Rabbit mAb

Journal:

Drug design, development and therapy

Application:

IHC, WB

IF:

4.8

Species:

Homo sapiens

PMID:

38681206

Title:

Validation of Core Ingredients and Molecular Mechanism of Cinobufotalin Injection Against Liver Cancer - PMC

References for [KO Validated] CDK4 Rabbit mAb (A11136)

Product:

[KO Validated] CDK4 Rabbit mAb

Journal:

Journal of cancer research and clinical oncology

Application:

IF:

3.6

Species:

Homo sapiens

PMID:

39052126

Title:

KIS, a target of SOX4, regulates the ID1-mediated enhancement of -catenin to facilitate lung adenocarcinoma cell proliferation and metastasis - PMC

Publishing research using A11136? Please let us know so that we can cite the reference in this datasheet.

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제품 > 항체 > 단일클론항체(mAb)

[KO Validated] CDK4 Rabbit mAb (A11136)

Overview

Background

Immunogen information

Applications

Documents

Certificate of Compliance

Research Area

[KO Validated] CDK4 Rabbit mAb images

Product Protocols

항체 (5)

Secondary Antibodies (26)