제품 > 항체 > 다중클론항체(pAb)

CDK4 Rabbit pAb (A0366)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using [KO Validated] CDK4 Rabbit pAb (A0366) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Western blot analysis of lysates from wild type (WT) and CDK4 knockout (KO) HeLa cells, using [KO Validated] CDK4 Rabbit pAb (A0366) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - CDK4 Rabbit pAb (A0366)

Immunofluorescence analysis of HeLa cells using CDK4 Rabbit pAb (A0366) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of MCF7 cells using CDK4 Rabbit pAb (A0366) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - CDK4 Rabbit pAb (A0366)

Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg CDK4 antibody (A0366). Western blot was performed from the immunoprecipitate using CDK4 antibody (A0366) at a dilution of 1:1000.

Review

Overview

Product name	CDK4 Rabbit pAb
Catalog No.	A0366
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 200-303 of human CDK4 (NP_000066.1).
Sequence	VGCIFAEMFRRKPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVSLPRGAFPPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKDEGNPE
Gene ID
Swiss Prot
Synonyms	CMM3; PSK-J3; CDK4
Calculated MW	34kDa
Observed MW	34kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	293T, HeLa, Mouse thymus, Mouse kidney
Cellular location	Cytoplasm, Membrane, Nucleus.
Customer validation	(H460,A549, Human lung cancer, Non-small-cell carcinoma) IHC(Homo sapiens, Mus musculus) WB(Mus musculus, Homo sapiens, Sus scrofa, Capra hircus, Other) IHC(Homo sapiens) IF(Homo sapiens, Other) WB(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

CDK4 Rabbit pAb images

Western blot - CDK4 Rabbit pAb (A0366)

Immunofluorescence - CDK4 Rabbit pAb (A0366)

Immunoprecipitation - CDK4 Rabbit pAb (A0366)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A0366

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Reviews List

Product Protocols

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Scientific reports

Application:

IHC

IF:

4.379

Species:

Homo sapiens

PMID:

26795955

Title:

Tsc1 deficiency impairs mammary development in mice by suppression of AKT, nuclear ERα, and cell-cycle-driving proteins

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

international journal of molecular medicine

Application:

IF:

4.101

Species:

Mus musculus

PMID:

29512695

Title:

SUMO2 modification of Aurora B and its impact on follicular development and atresia in the mouse ovary

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Cell Cycle

Application:

IF:

4.534

Species:

Mus musculus

PMID:

32816597

Title:

The PRC2 complex directly regulates the cell cycle and controls proliferation in skeletal muscle

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Autophagy

Application:

IF:

16.016

Species:

Homo sapiens

PMID:

32397800

Title:

An integrative multi-omics approach uncovers the regulatory role of CDK7 and CDK4 in autophagy activation induced by silica nanoparticles

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Journal of Virology

Application:

IF:

5.103

Species:

Homo sapiens

PMID:

27334596

Title:

BZLF1 Attenuates Transmission of Inflammatory Paracrine Senescence in Epstein-Barr Virus-Infected Cells by Downregulating Tumor Necrosis Factor Alpha.

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Cell Cycle

Application:

IF:

4.534

Species:

Sus scrofa

PMID:

29119857

Title:

miR-638 Inhibits immature Sertoli cell growth by indirectly inactivating PI3K/AKT pathway via SPAG1 gene.

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Biochem Biophys Res Commun

Application:

IF:

3.575

Species:

Mus musculus

PMID:

32873389

Title:

MicroRNA-31 regulates dental epithelial cell proliferation by targeting Satb2

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

J Anim Sci Biotechnol

Application:

IF:

5.032

Species:

Capra hircus

PMID:

33072314

Title:

Effects of miR-101-3p on goat granulosa cells in vitro and ovarian development in vivo via STC1

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Oncol Lett

Application:

IF:

2.967

Species:

Homo sapiens

PMID:

33240422

Title:

TERT promoter regulating melittin expression induces apoptosis and G 0/G 1 cell cycle arrest in esophageal carcinoma cells

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Cell Biol Toxicol

Application:

IF:

6.691

Species:

Homo sapiens

PMID:

33126266

Title:

Induction of cell cycle arrest and apoptosis by CPUC002 through stabilization of p53 and suppression of STAT3 signaling pathway in multiple myeloma

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Reprod Domest Anim

Application:

IF:

2.005

Species:

Sus scrofa

PMID:

33305371

Title:

AR regulates porcine immature Sertoli cell growth via binding to RNF4 and miR‐124a

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Medicina (Kaunas)

Application:

IF:

2.43

Species:

Homo sapiens

PMID:

33321738

Title:

Tectorigenin Inhibits Glioblastoma Proliferation by G0/G1 Cell Cycle Arrest

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Biochem Pharmacol

Application:

IF:

5.858

Species:

Homo sapiens

PMID:

34111425

Title:

Inhibition of Pim-2 kinase by LT-171-861 promotes DNA damage and exhibits enhanced lethal effects with PARP Inhibitor in multiple myeloma

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

World J Gastroenterol

Application:

IF:

5.74

Species:

Mus musculus

PMID:

33362373

Title:

Nimbolide inhibits tumor growth by restoring hepatic tight junction protein expression and reduced inflammation in an experimental hepatocarcinogenesis

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

J Cancer

Application:

IF:

4.207

Species:

Mus musculus

PMID:

34659548

Title:

Bai-He-Gu-Jin-Tang formula suppresses lung cancer via AKT/GSK3β/β-catenin and induces autophagy via the AMPK/mTORC1/ULK1 signaling pathway

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

DNA and cell biology

Application:

IF:

3.55

Species:

Homo sapiens

PMID:

35325570

Title:

LINC00982 Inhibits the Proliferation, Migration, and Invasion of Breast Cancer Cells Through the miR-765/DPF3 Axis

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

European journal of pharmacology

Application:

IF:

5.19

Species:

Mus musculus

PMID:

35526567

Title:

Sampsonione F suppresses adipogenesis via activating p53 pathway during the mitotic clonal expansion progression of adipocyte differentiation

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Anticancer research

Application:

IF:

2.43

Species:

Homo sapiens

PMID:

36854527

Title:

Hinokitiol Inhibits the Viability of Oral Squamous Carcinoma Cells by Inducing Apoptosis and Autophagy

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Oncology reports

Application:

IF:

4.13

Species:

Homo sapiens

PMID:

36929422

Title:

NONHSAG028908.3 sponges miR-34a-5p to promote growth of colorectal cancer via targeting ALDOA - PMC

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Cancer gene therapy

Application:

IF:

5.85

Species:

Homo sapiens

PMID:

36241701

Title:

VEZF1, destabilized by STUB1, affects cellular growth and metastasis of hepatocellular carcinoma by transcriptionally regulating PAQR4

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

J Cell Mol Med

Application:

WB, IHC

IF:

5.295

Species:

Homo sapiens

PMID:

33630417

Title:

Down-regulation of RCC1 sensitizes immunotherapy by up-regulating PD-L1 via p27 kip1 /CDK4 axis in non-small cell lung cancer

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

WB, IHC, IF

IF:

7.419

Species:

Homo sapiens

PMID:

30784916

Title:

Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo.

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Artificial cells, nanomedicine, and biotechnology

Application:

WB, IHC, IF

IF:

6.355

Species:

Homo sapiens

PMID:

31588803

Title:

Ribociclib (LEE011) suppresses cell proliferation and induces apoptosis of MDA-MB-231 by inhibiting CDK4/6-cyclin D-Rb-E2F pathway

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Cardiovascular drugs and therapy

Application:

IF:

3.94

Species:

Homo sapiens

PMID:

38363479

Title:

Isoimperatorin Inhibits Angiogenesis by Suppressing VEGFR2 Signaling Pathway

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Oncology reports

Application:

IF:

4.13

Species:

Homo sapiens

PMID:

38186304

Title:

CDC6 may serve as an indicator of lung adenocarcinoma prognosis and progression based on TCGA and GEO data mining and experimental analyses

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Sci Total Environ

Application:

WB, IF

IF:

10.753

Species:

Other

PMID:

34743874

Title:

Physiological and transcriptomic analyses reveal the toxicological mechanism and risk assessment of environmentally-relevant waterborne tetracycline exposure on

References for CDK4 Rabbit pAb (A0366)

Product:

CDK4 Rabbit pAb

Journal:

Cells

Application:

IF:

7.666

Species:

Mus musculus

PMID:

34440873