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[KO Validated] DNAJB1 Rabbit pAb (A5504)

KO/KDValidated

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] DNAJB1 Rabbit pAb (A5504)

Western blot analysis of lysates from wild type (WT) and DNAJB1 knockout (KO) 293T cells, using [KO Validated] DNAJB1 Rabbit pAb (A5504) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] DNAJB1 Rabbit pAb (A5504)

Western blot analysis of various lysates using [KO Validated] DNAJB1 Rabbit pAb (A5504) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] DNAJB1 Rabbit pAb (A5504)

Western blot analysis of lysates from 293T cells using [KO Validated] DNAJB1 Rabbit pAb(A5504) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - [KO Validated] DNAJB1 Rabbit pAb (A5504)

Immunofluorescence analysis of U2OS cells using [KO Validated] DNAJB1 Rabbit pAb (A5504). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] DNAJB1 Rabbit pAb (A5504)

Immunofluorescence analysis of C6 cells using [KO Validated] DNAJB1 Rabbit pAb (A5504) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] DNAJB1 Rabbit pAb (A5504)

Immunofluorescence analysis of HeLa cells using [KO Validated] DNAJB1 Rabbit pAb (A5504) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] DNAJB1 Rabbit pAb
Catalog No.A5504
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human DNAJB1 (NP_006136.1).
SequenceMGKDYYQTLGLARGASDEEIKRAYRRQALRYHPDKNKEPGAEEKFKEIAEAYDVLSDPRKREIFDRYGEEGLKGSGPSGGSGGGANGTSFSYTFHGDPHAMFAEFFGGRNPFDTFFGQRNGEEGMDIDDPFSGFPMGMGGFTNVNFGRSRSAQEPARKKQDPPVTHDLRVSLEEIYSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKKGWKEGTKITFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDGRTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLIIEFEVIFPERIPQTSRTVLEQVLPI
Gene ID
Swiss Prot
SynonymsHdj1; Sis1; HSPF1; Hsp40; RSPH16B; B1
Calculated MW38kDa
Observed MW38kDa/38KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHep G2, C2C12, Mouse testis, Rat testis, 293T
Cellular locationCytoplasm, Nucleus, nucleolus
Customer validation

WB(Mus musculus, Homo sapiens, Gallus gallus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - [KO Validated] DNAJB1 Rabbit pAb (A5504)}

    Western blot - [KO Validated] DNAJB1 Rabbit pAb (A5504)

    Western blot analysis of lysates from wild type (WT) and DNAJB1 knockout (KO) 293T cells, using [KO Validated] DNAJB1 Rabbit pAb (A5504) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - [KO Validated] DNAJB1 Rabbit pAb (A5504)}

    Western blot - [KO Validated] DNAJB1 Rabbit pAb (A5504)

    Western blot analysis of various lysates using [KO Validated] DNAJB1 Rabbit pAb (A5504) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates / proteins: 25 μg per lane.
    Blocking buffer: 3 % nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - [KO Validated] DNAJB1 Rabbit pAb (A5504)}

    Western blot - [KO Validated] DNAJB1 Rabbit pAb (A5504)

    Western blot analysis of lysates from 293T cells using [KO Validated] DNAJB1 Rabbit pAb(A5504) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - [KO Validated] DNAJB1 Rabbit pAb (A5504)}

    Immunofluorescence - [KO Validated] DNAJB1 Rabbit pAb (A5504)

    Immunofluorescence analysis of U2OS cells using [KO Validated] DNAJB1 Rabbit pAb (A5504). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - [KO Validated] DNAJB1 Rabbit pAb (A5504)}

    Immunofluorescence - [KO Validated] DNAJB1 Rabbit pAb (A5504)

    Immunofluorescence analysis of C6 cells using [KO Validated] DNAJB1 Rabbit pAb (A5504) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - [KO Validated] DNAJB1 Rabbit pAb (A5504)}

    Immunofluorescence - [KO Validated] DNAJB1 Rabbit pAb (A5504)

    Immunofluorescence analysis of HeLa cells using [KO Validated] DNAJB1 Rabbit pAb (A5504) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A5504? Please let us know so that we can cite the reference in this datasheet.

    항체 (4)

    Secondary Antibodies (26)