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[KO Validated] ECE1 Rabbit pAb (A5638)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] ECE1 Rabbit pAb (A5638)

Western blot analysis of lysates from wild type (WT) and ECE1 knockout (KO) HeLa cells, using [KO Validated] ECE1 Rabbit pAb (A5638) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - [KO Validated] ECE1 Rabbit pAb (A5638)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using ECE1 Rabbit pAb (A5638) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] ECE1 Rabbit pAb (A5638)

Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using ECE1 Rabbit pAb (A5638) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] ECE1 Rabbit pAb (A5638)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ECE1 Rabbit pAb (A5638) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] ECE1 Rabbit pAb (A5638)

Immunofluorescence analysis of L929 cells using ECE1 Rabbit pAb (A5638) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] ECE1 Rabbit pAb
Catalog No.A5638
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 670-770 of human ECE1 (NP_001388.1).
SequenceADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW
Gene ID
Swiss Prot
SynonymsECE; E1
Calculated MW87kDa
Observed MW130kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa
Cellular locationCell membrane, Single-pass type II membrane protein.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - [KO Validated] ECE1 Rabbit pAb (A5638)}

    Western blot - [KO Validated] ECE1 Rabbit pAb (A5638)

    Western blot analysis of lysates from wild type (WT) and ECE1 knockout (KO) HeLa cells, using [KO Validated] ECE1 Rabbit pAb (A5638) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunohistochemistry - [KO Validated] ECE1 Rabbit pAb (A5638)}

    Immunohistochemistry - [KO Validated] ECE1 Rabbit pAb (A5638)

    Immunohistochemistry analysis of paraffin-embedded Rat spleen using ECE1 Rabbit pAb (A5638) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - [KO Validated] ECE1 Rabbit pAb (A5638)}

    Immunohistochemistry - [KO Validated] ECE1 Rabbit pAb (A5638)

    Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using ECE1 Rabbit pAb (A5638) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - [KO Validated] ECE1 Rabbit pAb (A5638)}

    Immunohistochemistry - [KO Validated] ECE1 Rabbit pAb (A5638)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ECE1 Rabbit pAb (A5638) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunofluorescence - [KO Validated] ECE1 Rabbit pAb (A5638)}

    Immunofluorescence - [KO Validated] ECE1 Rabbit pAb (A5638)

    Immunofluorescence analysis of L929 cells using ECE1 Rabbit pAb (A5638) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)