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[KO Validated] GARS Rabbit mAb (A0651)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] GARS Rabbit mAb (A0651)

Western blot analysis of Jurkat, using GARS antibody (A0651) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - [KO Validated] GARS Rabbit mAb (A0651)

Western blot analysis of various lysates, using [KO Validated] GARS Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Western blot - [KO Validated] GARS Rabbit mAb (A0651)

Western blot analysis of extracts from wild type(WT) and GARS knockout (KO) 293T cells, using GARS antibody (A0651) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name[KO Validated] GARS Rabbit mAb
Catalog No.A0651
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0514
This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 640-739 of human GARS (P41250).
SequenceRHGVSHKVDDSSGSIGRRYARTDEIGVAFGVTIDFDTVNKTPHTATLRDRDSMRQIRAEISELPSIVQDLANGNITWADVEARYPLFEGQETGKKETIEE
Gene ID
Swiss Prot
SynonymsGARS; HMN5; CMT2D; DSMAV; GlyRS; HMN5A; SMAD1; SMAJI
Calculated MW83kDa
Observed MW83kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesJurkat, 293T, Hep G2, Mouse brain, Rat brain
Cellular locationCell projection, Cytoplasm, Mitochondrion, Axon

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - [KO Validated] GARS Rabbit mAb (A0651)}

    Western blot - [KO Validated] GARS Rabbit mAb (A0651)

    Western blot analysis of Jurkat, using GARS antibody (A0651) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Western blot - [KO Validated] GARS Rabbit mAb (A0651)}

    Western blot - [KO Validated] GARS Rabbit mAb (A0651)

    Western blot analysis of various lysates, using [KO Validated] GARS Rabbit mAb at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.
    ABclonal:Western blot - [KO Validated] GARS Rabbit mAb (A0651)}

    Western blot - [KO Validated] GARS Rabbit mAb (A0651)

    Western blot analysis of extracts from wild type(WT) and GARS knockout (KO) 293T cells, using GARS antibody (A0651) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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