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[KO Validated] HDAC8 Rabbit pAb (A5829)

KO/KDValidated

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] HDAC8 Rabbit pAb (A5829)

Western blot analysis of lysates from wild type (WT) and HDAC8 knockout (KO) HeLa cells, using [KO Validated] HDAC8 Rabbit pAb (A5829) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] HDAC8 Rabbit pAb (A5829)

Western blot analysis of lysates from C6 cells, using [KO Validated] HDAC8 Rabbit pAb (A5829) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - [KO Validated] HDAC8 Rabbit pAb (A5829)

Immunohistochemistry analysis of paraffin-embedded Rat testis using [KO Validated] HDAC8 Rabbit pAb (A5829) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] HDAC8 Rabbit pAb (A5829)

Immunohistochemistry analysis of paraffin-embedded Rat spinal cord using [KO Validated] HDAC8 Rabbit pAb (A5829) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] HDAC8 Rabbit pAb (A5829)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using [KO Validated] HDAC8 Rabbit pAb (A5829) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product name[KO Validated] HDAC8 Rabbit pAb
Catalog No.A5829
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human HDAC8 (NP_060956.1).
SequenceMEEPEEPADSGQSLVPVYIYSPEYVSMCDSLAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEGDDDHPDSIEYGLGYDCPATEGIFDYAAAIGGATITAAQCLIDGMCKVAINWSGGWHHAKKDEASGFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDVSDVGLGKGRYYSVNVPIQDGIQDEKYYQICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPDYVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVV
Gene ID
Swiss Prot
SynonymsHD8; WTS; RPD3; CDA07; CDLS5; KDAC8; MRXS6; HDACL1; C8
Calculated MW42kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesC6
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens, Mus musculus, Mus musculus , Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] HDAC8 Rabbit pAb (A5829)}

Western blot - [KO Validated] HDAC8 Rabbit pAb (A5829)

Western blot analysis of lysates from wild type (WT) and HDAC8 knockout (KO) HeLa cells, using [KO Validated] HDAC8 Rabbit pAb (A5829) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] HDAC8 Rabbit pAb (A5829)}

Western blot - [KO Validated] HDAC8 Rabbit pAb (A5829)

Western blot analysis of lysates from C6 cells, using [KO Validated] HDAC8 Rabbit pAb (A5829) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - [KO Validated] HDAC8 Rabbit pAb (A5829)}

Immunohistochemistry - [KO Validated] HDAC8 Rabbit pAb (A5829)

Immunohistochemistry analysis of paraffin-embedded Rat testis using [KO Validated] HDAC8 Rabbit pAb (A5829) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] HDAC8 Rabbit pAb (A5829)}

Immunohistochemistry - [KO Validated] HDAC8 Rabbit pAb (A5829)

Immunohistochemistry analysis of paraffin-embedded Rat spinal cord using [KO Validated] HDAC8 Rabbit pAb (A5829) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] HDAC8 Rabbit pAb (A5829)}

Immunohistochemistry - [KO Validated] HDAC8 Rabbit pAb (A5829)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using [KO Validated] HDAC8 Rabbit pAb (A5829) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A5829? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)