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[KO Validated] Hexokinase II Rabbit mAb (A20829)

KO/KDValidated

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] Hexokinase II Rabbit mAb (A20829)

Western blot analysis of various lysates, using Hexokinase II Rabbit mAb (A20829) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] Hexokinase II Rabbit mAb (A20829)

Western blot analysis of lysates from wild type(WT) and Hexokinase II knockout (KO) HeLa cells, using Hexokinase II Rabbit mAb (A20829) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name[KO Validated] Hexokinase II Rabbit mAb
Catalog No.A20829
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC52060
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Hexokinase II (NP_000180.2).
SequenceMIASHLLAYFFTELNHDQVQKVDQYLYHMRLSDETLLEISKRFRKEMEKGLGATTHPTAAVKMLPTFVRSTPDGTEHGEFLALDLGGTNFRVLWVKVTDNGLQKVEMENQIYAIPEDIMR
Gene ID
Swiss Prot
SynonymsHKII; HXK2; II
Calculated MW102kDa
Observed MW102kD
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, RD, SH-SY5Y, Mouse heart
Cellular locationMitochondrion outer membrane, Cytoplasm, cytosol.
Customer validation

WB(Mus musculus, Homo sapiens, Other)

IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] Hexokinase II Rabbit mAb (A20829)}

Western blot - [KO Validated] Hexokinase II Rabbit mAb (A20829)

Western blot analysis of various lysates, using Hexokinase II Rabbit mAb (A20829) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] Hexokinase II Rabbit mAb (A20829)}

Western blot - [KO Validated] Hexokinase II Rabbit mAb (A20829)

Western blot analysis of lysates from wild type(WT) and Hexokinase II knockout (KO) HeLa cells, using Hexokinase II Rabbit mAb (A20829) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A20829? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

ELISA 키트 (1)

Secondary Antibodies (25)