제품 > 항체 > 단일클론항체(mAb)

[KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from wild type (WT) and HuR/ELAVL1 knockout (KO) 293T cells, using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of various lysates using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human kidney using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)

Confocal imaging of NIH/3T3 cells using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Confocal imaging of U-2 OS cells using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

ABclonal:Immunoprecipitation - [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg HuR/ELAVL1 antibody (A19622). Western blot was performed from the immunoprecipitate using HuR/ELAVL1 antibody (A19622) at a dilution of 1:1000.

Overview

Product name	[KO Validated] HuR/ELAVL1 Rabbit mAb
Catalog No.	A19622
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0101

Background

The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR/ELAVL1 (Q15717).
Sequence	MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSS
Gene ID
Swiss Prot
Synonyms	HUR; Hua; MelG; ELAV1; L1
Calculated MW	36kDa
Observed MW	36kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:6000 IHC-P 1:200 - 1:2000 IF/ICC 1:100 - 1:1000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	293T, HeLa, Jurkat, Mouse brain, Mouse spleen, Mouse thymus, Rat brain
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Homo sapiens, Mus musculus) Co-IP(Homo sapiens) IP(Rattus norvegicus) RIP(Rattus norvegicus) RIP(Homo sapiens) IP(Homo sapiens) IF(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] HuR/ELAVL1 Rabbit mAb images

Western blot - [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)

Immunohistochemistry - [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)

Immunofluorescence - [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)

Immunoprecipitation - [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)

Product:

[KO Validated] HuR/ELAVL1 Rabbit mAb

Journal:

Mbio

Application:

IF:

7.867

Species:

Homo sapiens

PMID:

32156816

Title:

RNA Binding Motif Protein RBM45 Regulates Expression of the 11-Kilodalton Protein of Parvovirus B19 through Binding to Novel Intron Splicing Enhancers

References for [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)

Product:

[KO Validated] HuR/ELAVL1 Rabbit mAb

Journal:

Neuroreport

Application:

IF:

1.7

Species:

Mus musculus

PMID:

36367790

Title:

LncRNA Taurine Up-Regulated 1 plays a proapoptotic role by regulating nuclear-cytoplasmic shuttle of HuR under the condition of neuronal ischemia - PMC

References for [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)

Product:

[KO Validated] HuR/ELAVL1 Rabbit mAb

Journal:

Application:

IF:

Species:

Homo sapiens

PMID:

38067260

Title:

Circular RNA CDR1as Mediated by Human Antigen R (HuR) Promotes Gastric Cancer Growth via miR-299-3p/TGIF1 Axis - PMC

References for [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)

Product:

[KO Validated] HuR/ELAVL1 Rabbit mAb

Journal:

Virologica Sinica

Application:

IF:

6.94

Species:

Homo sapiens

PMID:

37543144

Title:

Long noncoding RNA 1392 regulates MDA5 by interaction with ELAVL1 to inhibit Coxsackievirus B5 infection

References for [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)

Product:

[KO Validated] HuR/ELAVL1 Rabbit mAb

Journal:

Oncogene

Application:

Co-IP

IF:

8.75

Species:

Homo sapiens

PMID:

38366146

Title:

CircUSP1 as a novel marker promotes gastric cancer progression via stabilizing HuR to upregulate USP1 and Vimentin

References for [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)

Product:

[KO Validated] HuR/ELAVL1 Rabbit mAb

Journal:

Redox biology

Application:

IP, RIP

IF:

10.78

Species:

Rattus norvegicus

PMID:

38316068

Title:

FTO ameliorates doxorubicin-induced cardiotoxicity by inhibiting ferroptosis via P53-P21/Nrf2 activation in a HuR-dependent m6A manner - PMC

References for [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)

Product:

[KO Validated] HuR/ELAVL1 Rabbit mAb

Journal:

Cellular signalling

Application:

RIP

IF:

4.8

Species:

Homo sapiens

PMID:

38387687

Title:

LncRNA HOST2 promotes NSUN2-mediated breast cancer progression via interaction with ELAVL1

References for [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)

Product:

[KO Validated] HuR/ELAVL1 Rabbit mAb

Journal:

Cellular signalling

Application:

IF:

4.8

Species:

Homo sapiens

PMID:

38387687

Title:

LncRNA HOST2 promotes NSUN2-mediated breast cancer progression via interaction with ELAVL1

References for [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)

Product:

[KO Validated] HuR/ELAVL1 Rabbit mAb

Journal:

Journal of experimental & clinical cancer research : CR

Application:

WB, IP

IF:

Species:

Homo sapiens

PMID:

36171592

Title:

LncRNA FAM83H-AS1 promotes the malignant progression of pancreatic ductal adenocarcinoma by stabilizing FAM83H mRNA to protect -catenin from degradation - PMC

References for [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)

Product:

[KO Validated] HuR/ELAVL1 Rabbit mAb

Journal:

Purinergic signalling

Application:

IF:

3.5

Species:

Homo sapiens

PMID:

38801618

Title:

Molecular mechanisms of extracellular-ATP-mediated colorectal cancer progression: Implication of purinergic receptors-mediated nucleocytoplasmic shuttling of HuR