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[KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)

Western blot analysis of various lysates using [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Western blot - [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)

Western blot analysis of lysates from wild type (WT) and HuR/ELAVL1 knockout (KO) 293T cells, using [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)

Immunohistochemistry analysis of paraffin-embedded mouse testis using [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)

Immunohistochemistry analysis of paraffin-embedded rat testis using [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)

Immunofluorescence analysis of MCF-7 cells using [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] HuR/ELAVL1 Rabbit pAb
Catalog No.A1608
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human HuR/ELAVL1 (NP_001410.2).
SequenceMSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNASSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTNKSHK
Gene ID
Swiss Prot
SynonymsHUR; Hua; MelG; ELAV1; L1
Calculated MW36kDa
Observed MW37kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:100 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-251MG, HeLa, Jurkat, K-562, PC-3
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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ABclonal:Western blot - [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)}

Western blot - [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)

Western blot analysis of various lysates using [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)}

Western blot - [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)

Western blot analysis of lysates from wild type (WT) and HuR/ELAVL1 knockout (KO) 293T cells, using [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)}

Immunohistochemistry - [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)

Immunohistochemistry analysis of paraffin-embedded mouse testis using [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)}

Immunohistochemistry - [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)

Immunohistochemistry analysis of paraffin-embedded rat testis using [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)}

Immunohistochemistry - [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)}

Immunofluorescence - [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608)

Immunofluorescence analysis of MCF-7 cells using [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)