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[KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)

KO/KDValidated

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)

Western blot analysis of lysates from wild type (WT) and PKA RIIα (PRKAR2A)/PKR2 knockout (KO) HeLa cells, using [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)

Western blot analysis of various lysates using [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)

Immunofluorescence analysis of L929 cells using [KO Validated] PKA RIIα (PRKAR2A)/PKR2 Rabbit pAb (A1531) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb
Catalog No.A1531
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human PKA RIIα (PRKAR2A)/PKR2 (NP_004148.1).
SequenceMSHIQIPPGLTELLQGYTVEVLRQQPPDLVEFAVEYFTRLREARAPASVLPAATPRQSLGHPPPEPGPDRVADAKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQGEKADSFYIIESGEVSILIRSRTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ
Gene ID
Swiss Prot
SynonymsPKR2; PRKAR2; A)
Calculated MW46kDa
Observed MW46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, Jurkat, SW480, LO2, Mouse lung, Rat skeletal muscle
Cellular locationCell membrane, Cytoplasm.
Customer validation

IHC(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)}

Western blot - [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)

Western blot analysis of lysates from wild type (WT) and PKA RIIα (PRKAR2A)/PKR2 knockout (KO) HeLa cells, using [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)}

Western blot - [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)

Western blot analysis of various lysates using [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)}

Immunofluorescence - [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (A1531)

Immunofluorescence analysis of L929 cells using [KO Validated] PKA RIIα (PRKAR2A)/PKR2 Rabbit pAb (A1531) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1531? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)