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Phospho-PKA RIIα (PRKAR2A)-S99 Rabbit mAb (AP1034)

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Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - Phospho-PKA RIIα (PRKAR2A)-S99 Rabbit mAb (AP1034)

Western blot analysis of lysates from 293T cells, using Phospho-PKA RIIα (PRKAR2A)-S99 Rabbit mAb (AP1034) at 1:1000 dilution. 293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - Phospho-PKA RIIα (PRKAR2A)-S99 Rabbit mAb (AP1034)

Western blot analysis of lysates from C6 cells, using Phospho-PKA RIIα (PRKAR2A)-S99 Rabbit mAb (AP1034) at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

Overview

Product namePhospho-PKA RIIα (PRKAR2A)-S99 Rabbit mAb
Catalog No.AP1034
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1579
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
ImmunogenA synthetic phosphorylated peptide around S99 of human PRKAR2A/PKR2 (P13861).
SequenceRVSVC
Gene ID
Swiss Prot
SynonymsPKR2; PRKAR2; Phospho-PKA RIIα (PRKAR2A)-S99
Calculated MW46kDa
Observed MW51kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, C6
Cellular locationCell membrane, Cytoplasm.

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ABclonal:Western blot - Phospho-PKA RIIα (PRKAR2A)-S99 Rabbit mAb (AP1034)}

Western blot - Phospho-PKA RIIα (PRKAR2A)-S99 Rabbit mAb (AP1034)

Western blot analysis of lysates from 293T cells, using Phospho-PKA RIIα (PRKAR2A)-S99 Rabbit mAb (AP1034) at 1:1000 dilution. 293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - Phospho-PKA RIIα (PRKAR2A)-S99 Rabbit mAb (AP1034)}

Western blot - Phospho-PKA RIIα (PRKAR2A)-S99 Rabbit mAb (AP1034)

Western blot analysis of lysates from C6 cells, using Phospho-PKA RIIα (PRKAR2A)-S99 Rabbit mAb (AP1034) at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)