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PKA RIIα (PRKAR2A) Rabbit mAb (A3889)

Publications (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - PKA RIIα (PRKAR2A) Rabbit mAb (A3889)

Western blot analysis of extracts of various cell lines, using PKA RIIα (PRKAR2A) Rabbit mAb (A3889) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Overview

Product namePKA RIIα (PRKAR2A) Rabbit mAb
Catalog No.A3889
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0860
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 312-404 of human PKA RIIα (PRKAR2A) (P13861).
SequenceSRTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ
Gene ID
Swiss Prot
SynonymsPKR2
Calculated MW46kDa
Observed MW50kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, HepG2
Cellular locationCell membrane, Cytoplasm
Customer validation

WB(Rattus norvegicus)

Documents

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ABclonal:Western blot - PKA RIIα (PRKAR2A) Rabbit mAb (A3889)}

Western blot - PKA RIIα (PRKAR2A) Rabbit mAb (A3889)

Western blot analysis of extracts of various cell lines, using PKA RIIα (PRKAR2A) Rabbit mAb (A3889) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A3889? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)