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[KO Validated] Rap1A Rabbit pAb (A0975)

KO/KDValidated

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] Rap1A Rabbit pAb (A0975)

Western blot analysis of various lysates using [KO Validated] Rap1A Rabbit pAb (A0975) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] Rap1A Rabbit pAb (A0975)

Western blot analysis of lysates from wild type(WT) and Rap1A knockdown (KD) 293T cells, using [KO Validated] Rap1A Rabbit pAb (A0975) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KO Validated] Rap1A Rabbit pAb (A0975)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] Rap1A Rabbit pAb (A0975) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] Rap1A Rabbit pAb (A0975)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] Rap1A Rabbit pAb (A0975) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] Rap1A Rabbit pAb (A0975)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using [KO Validated] Rap1A Rabbit pAb (A0975) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product name[KO Validated] Rap1A Rabbit pAb
Catalog No.A0975
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 39-138 of human Rap1A (NP_002875.1).
SequenceSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQW
Gene ID
Swiss Prot
SynonymsRAP1; C21KG; G-22K; KREV1; KREV-1; SMGP21; 1A
Calculated MW21kDa
Observed MW21kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, U-87MG, HL-60
Cellular locationCell junction, Cell membrane, Cytoplasm, Early endosome, Lipid-anchor, perinuclear region.
Customer validation

WB(Rattus norvegicus, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KO Validated] Rap1A Rabbit pAb (A0975)}

Western blot - [KO Validated] Rap1A Rabbit pAb (A0975)

Western blot analysis of various lysates using [KO Validated] Rap1A Rabbit pAb (A0975) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] Rap1A Rabbit pAb (A0975)}

Western blot - [KO Validated] Rap1A Rabbit pAb (A0975)

Western blot analysis of lysates from wild type(WT) and Rap1A knockdown (KD) 293T cells, using [KO Validated] Rap1A Rabbit pAb (A0975) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] Rap1A Rabbit pAb (A0975)}

Immunohistochemistry - [KO Validated] Rap1A Rabbit pAb (A0975)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] Rap1A Rabbit pAb (A0975) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] Rap1A Rabbit pAb (A0975)}

Immunohistochemistry - [KO Validated] Rap1A Rabbit pAb (A0975)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] Rap1A Rabbit pAb (A0975) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] Rap1A Rabbit pAb (A0975)}

Immunohistochemistry - [KO Validated] Rap1A Rabbit pAb (A0975)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using [KO Validated] Rap1A Rabbit pAb (A0975) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A0975? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)