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[KO Validated] SIRT5 Rabbit pAb (A5784)

KO/KDValidated

Publication (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] SIRT5 Rabbit pAb (A5784)

Western blot analysis of lysates from wild type (WT) and SIRT5 knockout (KO) 293T cells, using [KO Validated] SIRT5 Rabbit pAb (A5784) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] SIRT5 Rabbit pAb (A5784)

Western blot analysis of various lysates, using [KO Validated] SIRT5 Rabbit pAb (A5784) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] SIRT5 Rabbit pAb (A5784)

Western blot analysis of various lysates, using [KO Validated] SIRT5 Rabbit pAb (A5784) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product name[KO Validated] SIRT5 Rabbit pAb
Catalog No.A5784
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 37-310 of human SIRT5 (NP_036373.1).
SequenceSSSMADFRKFFAKAKHIVIISGAGVSAESGVPTFRGAGGYWRKWQAQDLATPLAFAHNPSRVWEFYHYRREVMGSKEPNAGHRAIAECETRLGKQGRRVVVITQNIDELHRKAGTKNLLEIHGSLFKTRCTSCGVVAENYKSPICPALSGKGAPEPGTQDASIPVEKLPRCEEAGCGGLLRPHVVWFGENLDPAILEEVDRELAHCDLCLVVGTSSVVYPAAMFAPQVAARGVPVAEFNTETTPATNRFRFHFQGPCGTTLPEALACHENETVS
Gene ID
Swiss Prot
SynonymsSIR2L5; T5
Calculated MW34kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHCT 116, 293F, Mouse heart, Mouse liver, Rat heart, Rat liver
Cellular locationCytoplasm, Mitochondrion, Mitochondrion intermembrane space, Mitochondrion matrix, Nucleus, cytosol
Customer validation

WB(Homo sapiens)

IP(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - [KO Validated] SIRT5 Rabbit pAb (A5784)}

    Western blot - [KO Validated] SIRT5 Rabbit pAb (A5784)

    Western blot analysis of lysates from wild type (WT) and SIRT5 knockout (KO) 293T cells, using [KO Validated] SIRT5 Rabbit pAb (A5784) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - [KO Validated] SIRT5 Rabbit pAb (A5784)}

    Western blot - [KO Validated] SIRT5 Rabbit pAb (A5784)

    Western blot analysis of various lysates, using [KO Validated] SIRT5 Rabbit pAb (A5784) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - [KO Validated] SIRT5 Rabbit pAb (A5784)}

    Western blot - [KO Validated] SIRT5 Rabbit pAb (A5784)

    Western blot analysis of various lysates, using [KO Validated] SIRT5 Rabbit pAb (A5784) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A5784? Please let us know so that we can cite the reference in this datasheet.

    항체 (2)

    Secondary Antibodies (26)