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SIRT5 Rabbit pAb (A21827)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - SIRT5 Rabbit pAb (A21827)

Western blot analysis of extracts of various cell lines, using SIRT5 antibody (A21827) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product nameSIRT5 Rabbit pAb
Catalog No.A21827
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 216-310 of human SIRT5 (NP_036373.1).
SequenceLRPHVVWFGENLDPAILEEVDRELAHCDLCLVVGTSSVVYPAAMFAPQVAARGVPVAEFNTETTPATNRFRFHFQGPCGTTLPEALACHENETVS
Gene ID
Swiss Prot
SynonymsSIR2L5
Calculated MW34kDa
Observed MW30kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHCT116, HepG2, SKOV3
Cellular locationCytoplasm, Mitochondrion, Mitochondrion intermembrane space, Mitochondrion matrix, Nucleus, cytosol

Documents

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    ABclonal:Western blot - SIRT5 Rabbit pAb (A21827)}

    Western blot - SIRT5 Rabbit pAb (A21827)

    Western blot analysis of extracts of various cell lines, using SIRT5 antibody (A21827) at 1:500 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (2)

    Secondary Antibodies (26)