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[KO Validated] SMARCB1/SNF5 Rabbit mAb (A23466)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - [KO Validated] SMARCB1/SNF5 Rabbit mAb (A23466)

Western blot analysis of various lysates using SMARCB1/SNF5 Rabbit mAb (A23466) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] SMARCB1/SNF5 Rabbit mAb (A23466)

Western blot analysis of various lysates using SMARCB1/SNF5 Rabbit mAb (A23466) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] SMARCB1/SNF5 Rabbit mAb (A23466)

Western blot analysis of lysates from wild type (WT) and SMARCB1/SNF5 knockout (KO) 293T cells, using [KO Validated] SMARCB1/SNF5 Rabbit mAb (A23466) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - [KO Validated] SMARCB1/SNF5 Rabbit mAb (A23466)

Immunofluorescence analysis of NIH-3T3 cells using SMARCB1/SNF5 Rabbit mAb (A23466) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] SMARCB1/SNF5 Rabbit mAb (A23466)

Immunofluorescence analysis of U-2 OS cells using SMARCB1/SNF5 Rabbit mAb (A23466) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] SMARCB1/SNF5 Rabbit mAb
Catalog No.A23466
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0766
The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Alternatively spliced transcript variants have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 275-375 of human SMARCB1/SNF5 (NP_003064.2).
SequenceLVDQFEWDMSEKENSPEKFALKLCSELGLGGEFVTTIAYSIRGQLSWHQKTYAFSENPLPTVEIAIRNTGDADQWCPLLETLTDAEMEKKIRDQDRNTRRM
Gene ID
Swiss Prot
SynonymsRDT; CSS3; INI1; SNF5; Snr1; BAF47; INI-1; MRD15; RTPS1; Sfh1p; hSNFS; SNF5L1; SWNTS1; PPP1R144; F5
Calculated MW44kDa
Observed MW44kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500-1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, K-562, 293T WT, Mouse spinal cord, Mouse brain
Cellular locationNucleus

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] SMARCB1/SNF5 Rabbit mAb (A23466)}

Western blot - [KO Validated] SMARCB1/SNF5 Rabbit mAb (A23466)

Western blot analysis of various lysates using SMARCB1/SNF5 Rabbit mAb (A23466) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] SMARCB1/SNF5 Rabbit mAb (A23466)}

Western blot - [KO Validated] SMARCB1/SNF5 Rabbit mAb (A23466)

Western blot analysis of various lysates using SMARCB1/SNF5 Rabbit mAb (A23466) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] SMARCB1/SNF5 Rabbit mAb (A23466)}

Western blot - [KO Validated] SMARCB1/SNF5 Rabbit mAb (A23466)

Western blot analysis of lysates from wild type (WT) and SMARCB1/SNF5 knockout (KO) 293T cells, using [KO Validated] SMARCB1/SNF5 Rabbit mAb (A23466) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - [KO Validated] SMARCB1/SNF5 Rabbit mAb (A23466)}

Immunofluorescence - [KO Validated] SMARCB1/SNF5 Rabbit mAb (A23466)

Immunofluorescence analysis of NIH-3T3 cells using SMARCB1/SNF5 Rabbit mAb (A23466) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] SMARCB1/SNF5 Rabbit mAb (A23466)}

Immunofluorescence - [KO Validated] SMARCB1/SNF5 Rabbit mAb (A23466)

Immunofluorescence analysis of U-2 OS cells using SMARCB1/SNF5 Rabbit mAb (A23466) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

Secondary Antibodies (26)