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[KO Validated] SND1 Rabbit mAb (A25813)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] SND1 Rabbit mAb (A25813)

Western blot analysis of lysates from wild type (WT) and SND1 knockout (KO) HeLa cells using [KO Validated] SND1 Rabbit mAb (A25813) at 1:3700 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] SND1 Rabbit mAb (A25813)

Western blot analysis of lysates from Jurkat cells using [KO Validated] SND1 Rabbit mAb (A25813) at 1:3700 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - [KO Validated] SND1 Rabbit mAb (A25813)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KO Validated] SND1 Rabbit mAb (A25813) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] SND1 Rabbit mAb (A25813)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KO Validated] SND1 Rabbit mAb (A25813) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] SND1 Rabbit mAb (A25813)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KO Validated] SND1 Rabbit mAb (A25813) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] SND1 Rabbit mAb (A25813)

Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using [KO Validated] SND1 Rabbit mAb (A25813) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] SND1 Rabbit mAb (A25813)

Confocal imaging of HeLa cells using [KO Validated] SND1 Rabbit mAb (A25813, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KO Validated] SND1 Rabbit mAb (A25813)

Confocal imaging of NIH/3T3 cells using [KO Validated] SND1 Rabbit mAb (A25813, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product name[KO Validated] SND1 Rabbit mAb
Catalog No.A25813
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC68115
This gene encodes a transcriptional co-activator that interacts with the acidic domain of Epstein-Barr virus nuclear antigen 2 (EBNA 2), a transcriptional activator that is required for B-lymphocyte transformation. Other transcription factors that interact with this protein are signal transducers and activators of transcription, STATs. This protein is also thought to be essential for normal cell growth. A similar protein in mammals and other organisms is a component of the RNA-induced silencing complex (RISC).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 26-285 of human SND1 (NP_055205.2).
SequenceMVLSGCAIIVRGQPRGGPPPERQINLSNIRAGNLARRAAATQPDAKDTPDEPWAFPAREFLRKKLIGKEVCFTIENKTPQGREYGMIYLGKDTNGENIAESLVAEGLATRREGMRANNPEQNRLSECEEQAKAAKKGMWSEGNGSHTIRDLKYTIENPRHFVDSHHQKPVNAIIEHVRDGSVVRALLLPDYYLVTVMLSGIKCPTFRREADGSETPEPFAAEAKFFTESRLLQRDVQIILESCHNQNILGTILHPNGNIT
Gene ID
Swiss Prot
Synonymsp100; TDRD11; Tudor-SN
Calculated MW102kDa
Observed MW102kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:3000 - 1:18000
  • IHC-P 1:500 - 1:5000
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Jurkat
Cellular locationCytoplasm, Melanosome, Nucleus.

Documents

Certificate of Compliance

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ABclonal:Western blot - [KO Validated] SND1 Rabbit mAb (A25813)}

Western blot - [KO Validated] SND1 Rabbit mAb (A25813)

Western blot analysis of lysates from wild type (WT) and SND1 knockout (KO) HeLa cells using [KO Validated] SND1 Rabbit mAb (A25813) at 1:3700 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] SND1 Rabbit mAb (A25813)}

Western blot - [KO Validated] SND1 Rabbit mAb (A25813)

Western blot analysis of lysates from Jurkat cells using [KO Validated] SND1 Rabbit mAb (A25813) at 1:3700 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] SND1 Rabbit mAb (A25813)}

Immunohistochemistry - [KO Validated] SND1 Rabbit mAb (A25813)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KO Validated] SND1 Rabbit mAb (A25813) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] SND1 Rabbit mAb (A25813)}

Immunohistochemistry - [KO Validated] SND1 Rabbit mAb (A25813)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KO Validated] SND1 Rabbit mAb (A25813) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] SND1 Rabbit mAb (A25813)}

Immunohistochemistry - [KO Validated] SND1 Rabbit mAb (A25813)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KO Validated] SND1 Rabbit mAb (A25813) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] SND1 Rabbit mAb (A25813)}

Immunohistochemistry - [KO Validated] SND1 Rabbit mAb (A25813)

Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using [KO Validated] SND1 Rabbit mAb (A25813) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KO Validated] SND1 Rabbit mAb (A25813)}

Immunofluorescence - [KO Validated] SND1 Rabbit mAb (A25813)

Confocal imaging of HeLa cells using [KO Validated] SND1 Rabbit mAb (A25813, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - [KO Validated] SND1 Rabbit mAb (A25813)}

Immunofluorescence - [KO Validated] SND1 Rabbit mAb (A25813)

Confocal imaging of NIH/3T3 cells using [KO Validated] SND1 Rabbit mAb (A25813, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

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