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[KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

KO/KDValidated

Publications (9) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Western blot analysis of various lysates using [KO Validated] SQSTM1/p62 Rabbit pAb (A11250) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Western blot analysis of lysates from wild type (WT) and SQSTM1 knockout (KO) 293T cells using [KO Validated] SQSTM1/p62 Rabbit pAb (A11250) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] SQSTM1/p62 Rabbit pAb (A11250) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Immunofluorescence analysis of U2OS cells using [KO Validated] SQSTM1/p62 Rabbit pAb (A11250) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Immunoprecipitation analysis of 200 μg extracts of C6 cells using 3 μg SQSTM1/p62 antibody (A11250). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (A11250) at a dilution of 1:1000.

Overview

Product name[KO Validated] SQSTM1/p62 Rabbit pAb
Catalog No.A11250
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1/p62 (NP_003891.1).
SequenceMASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL
Gene ID
Swiss Prot
Synonymsp60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3; 62
Calculated MW48kDa
Observed MW62kDa/
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:20 - 1:50
  • IF/ICC 1:20 - 1:50
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293T, HeLa, C6
Cellular locationCytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Late endosome, Lysosome, Nucleus, P-body, autophagosome.
Customer validation

WB(Homo sapiens, Rattus norvegicus, Mus musculus)

IHC(Rattus norvegicus)

IHC(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)}

Western blot - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Western blot analysis of various lysates using [KO Validated] SQSTM1/p62 Rabbit pAb (A11250) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)}

Western blot - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Western blot analysis of lysates from wild type (WT) and SQSTM1 knockout (KO) 293T cells using [KO Validated] SQSTM1/p62 Rabbit pAb (A11250) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)}

Immunohistochemistry - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] SQSTM1/p62 Rabbit pAb (A11250) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)}

Immunofluorescence - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Immunofluorescence analysis of U2OS cells using [KO Validated] SQSTM1/p62 Rabbit pAb (A11250) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)}

Immunoprecipitation - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Immunoprecipitation analysis of 200 μg extracts of C6 cells using 3 μg SQSTM1/p62 antibody (A11250). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (A11250) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A11250? Please let us know so that we can cite the reference in this datasheet.

항체 (4)

SQSTM1/p62 Rabbit mAb
A19700

WBIHC-PIF/ICCIPELISA

Reactivity:Human, Mouse, Rat

SQSTM1/p62 Rabbit mAb

SQSTM1/p62 Rabbit pAb
A7758

ELISAWBIHC-PIF/ICC

Reactivity:Human, Mouse, Rat

SQSTM1/p62 Rabbit pAb

SQSTM1/p62 Rabbit pAb
A11483

WBIHC-PIF/ICCIPELISA

Reactivity:Human, Mouse, Rat

SQSTM1/p62 Rabbit pAb

Phospho-SQSTM1/p62-S349 Rabbit pAb
A20737

ELISAWB

Reactivity:Human, Mouse, Rat

Phospho-SQSTM1/p62-S349 Rabbit pAb

Secondary Antibodies (25)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb