제품 > 항체 > 단일클론항체(mAb)

[KO Validated] Ythdc1 Rabbit mAb (A25682)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from Ythdc1-cKO mouse ES cells, using [KO Validated] Ythdc1 Rabbit mAb (A25682) at 1:1000 dilution incubated overnight at 4℃. Ythdc1-cKO mouse ES cells were treated by 4OHT.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

Western blot analysis of lysates from HeLa cells using [KO Validated] Ythdc1 Rabbit mAb (A25682) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

Review

Overview

Product name	[KO Validated] Ythdc1 Rabbit mAb
Catalog No.	A25682
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC66394

Background

Predicted to enable N6-methyladenosine-containing RNA binding activity and mRNA binding activity. Involved in posttranscriptional regulation of gene expression. Acts upstream of or within gamete generation; in utero embryonic development; and regulation of mRNA processing. Located in nucleus. Orthologous to human YTHDC1 (YTH domain containing 1).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 597-736 of mouse Ythdc1 (NP_808348.2).
Sequence	SYNDYVREFHNMGPPPPWQGMPPYPGIEQPPHHPYYQHHAPPPQAHPPYSGHHPVPHEARYRDKRVHDYDMRVDDFLRRTQAVVSGRRSRPRERDRERERDRPRDNRRDRERDRGRDRERERERICDRDRDRGERGRYRR
Gene ID
Swiss Prot
Synonyms	mKIAA1966; A730098D12Rik; Mouse Ythdc1
Calculated MW	85kDa
Observed MW	100kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Mouse ES cells, HeLa
Cellular location	Nucleus. Localizes to a novel subnuclear structure, the YT bodies.
Customer validation	(Embryonic Stem Cells)