제품 > 항체 > 다중클론항체(pAb)

Mouse Ythdc1 Rabbit pAb (A24548)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of lysates from NIH/3T3 cells, using Mouse Ythdc1 Rabbit pAb (A24548) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product name	Mouse Ythdc1 Rabbit pAb
Catalog No.	A24548
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Predicted to enable N6-methyladenosine-containing RNA binding activity and mRNA binding activity. Involved in posttranscriptional regulation of gene expression. Acts upstream of or within gamete generation; in utero embryonic development; and regulation of mRNA processing. Located in nucleus. Orthologous to human YTHDC1 (YTH domain containing 1).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 597-736 of mouse Ythdc1 (NP_808348.2).
Sequence	SYNDYVREFHNMGPPPPWQGMPPYPGIEQPPHHPYYQHHAPPPQAHPPYSGHHPVPHEARYRDKRVHDYDMRVDDFLRRTQAVVSGRRSRPRERDRERERDRPRDNRRDRERDRGRDRERERERICDRDRDRGERGRYRR
Gene ID
Swiss Prot
Synonyms	mKIAA1966; A730098D12Rik; Mouse Ythdc1
Calculated MW	85kDa
Observed MW	110kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	NIH/3T3
Cellular location	Nucleus. Localizes to a novel subnuclear structure, the YT bodies.