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[KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733)

Western blot analysis of various lysates using [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733)

Western blot analysis of lysates from wild type(WT) and hnRNP E1/PCBP1 knockout (KO) HeLa cells, using [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using hnRNP E1/PCBP1 Rabbit pAb (A21733) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733)

Immunohistochemistry analysis of paraffin-embedded mouse testis using hnRNP E1/PCBP1 Rabbit pAb (A21733) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733)

Immunohistochemistry analysis of paraffin-embedded rat brain using hnRNP E1/PCBP1 Rabbit pAb (A21733) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product name[KO Validated] hnRNP E1/PCBP1 Rabbit pAb
Catalog No.A21733
Host speciesrabbit
Purification methodAffinity purification
IsotypeIgG
This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human hnRNP E1/PCBP1 (NP_006187.2).
SequenceLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDR
Gene ID
Swiss Prot
SynonymsHNRPX; HNRPE1; hnRNP-X; HEL-S-85; hnRNP-E1; P1
Calculated MW37kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHepG2, NIH/3T3, Rat pancreas, HeLa
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733)}

Western blot - [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733)

Western blot analysis of various lysates using [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733)}

Western blot - [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733)

Western blot analysis of lysates from wild type(WT) and hnRNP E1/PCBP1 knockout (KO) HeLa cells, using [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733)}

Immunohistochemistry - [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using hnRNP E1/PCBP1 Rabbit pAb (A21733) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733)}

Immunohistochemistry - [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733)

Immunohistochemistry analysis of paraffin-embedded mouse testis using hnRNP E1/PCBP1 Rabbit pAb (A21733) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733)}

Immunohistochemistry - [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (A21733)

Immunohistochemistry analysis of paraffin-embedded rat brain using hnRNP E1/PCBP1 Rabbit pAb (A21733) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

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Secondary Antibodies (26)