제품 > 항체 > 단일클론항체(mAb)

hnRNP E1/PCBP1 Rabbit mAb (A22141)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - hnRNP E1/PCBP1 Rabbit mAb (A22141)

Western blot analysis of mouse brain, using hnRNP E1/PCBP1 Rabbit mAb (A22141) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - hnRNP E1/PCBP1 Rabbit mAb (A22141)

Western blot analysis of HeLa cells, using hnRNP E1/PCBP1 Rabbit mAb (A22141) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Overview

Product namehnRNP E1/PCBP1 Rabbit mAb
Catalog No.A22141
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55411
This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human hnRNP E1/PCBP1 (NP_006187.2).
SequenceLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDR
Gene ID
Swiss Prot
SynonymsHNRPX; HNRPE1; hnRNP-X; HEL-S-85; hnRNP-E1
Calculated MW37kDa
Observed MW38kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000-1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, Mouse brain
Cellular locationcytoplasm, cytoplasmic ribonucleoprotein granule, cytosol, extracellular exosome, nuclear speck, nucleoplasm, nucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - hnRNP E1/PCBP1 Rabbit mAb (A22141)}

Western blot - hnRNP E1/PCBP1 Rabbit mAb (A22141)

Western blot analysis of mouse brain, using hnRNP E1/PCBP1 Rabbit mAb (A22141) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - hnRNP E1/PCBP1 Rabbit mAb (A22141)}

Western blot - hnRNP E1/PCBP1 Rabbit mAb (A22141)

Western blot analysis of HeLa cells, using hnRNP E1/PCBP1 Rabbit mAb (A22141) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

재조합 단백질 (1)

Secondary Antibodies (26)