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KPNB1 Rabbit mAb (A23235)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KPNB1 Rabbit mAb (A23235)

Western blot analysis of various lysates, using KPNB1 Rabbit mAb (A23235) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - KPNB1 Rabbit mAb (A23235)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using KPNB1 Rabbit mAb (A23235) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - KPNB1 Rabbit mAb (A23235)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using KPNB1 Rabbit mAb (A23235) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - KPNB1 Rabbit mAb (A23235)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using KPNB1 Rabbit mAb (A23235) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - KPNB1 Rabbit mAb (A23235)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using KPNB1 Rabbit mAb (A23235) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameKPNB1 Rabbit mAb
Catalog No.A23235
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC59625
Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300-400 of human KPNB1. (NP_002256.2).
SequenceAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGCILEGPEPS
Gene ID
Swiss Prot
SynonymsIMB1; IPO1; IPOB; Impnb; NTF97; KPNB1
Calculated MW97kDa
Observed MW97kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesK562, HeLa, NIH/3T3, Mouse placenta, Mouse stomach (低表达) , C6, PC-12
Cellular locationCytoplasm, Nucleus envelope

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ABclonal:Western blot - KPNB1 Rabbit mAb (A23235)}

Western blot - KPNB1 Rabbit mAb (A23235)

Western blot analysis of various lysates, using KPNB1 Rabbit mAb (A23235) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - KPNB1 Rabbit mAb (A23235)}

Immunohistochemistry - KPNB1 Rabbit mAb (A23235)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using KPNB1 Rabbit mAb (A23235) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - KPNB1 Rabbit mAb (A23235)}

Immunohistochemistry - KPNB1 Rabbit mAb (A23235)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using KPNB1 Rabbit mAb (A23235) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - KPNB1 Rabbit mAb (A23235)}

Immunohistochemistry - KPNB1 Rabbit mAb (A23235)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using KPNB1 Rabbit mAb (A23235) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - KPNB1 Rabbit mAb (A23235)}

Immunohistochemistry - KPNB1 Rabbit mAb (A23235)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using KPNB1 Rabbit mAb (A23235) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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